International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3539-3545

Screening, Characterization and Identification of Probiotic Potential LAB with Phytase Activity
R.M. Dhingani*, B.H. Joshi and R.V. Prasad
Department of Food Quality Assurance, College of Food Processing Technology and Bio-Energy, Anand Agricultural University, Anand-388110, India
*Corresponding author

Probiotics have been extensively studied and explored commercially in many different products in the world. Looking to the demand and health prospects extensive screening for Lactic Acid Bacteria (LAB) with probiotic potentials was investigated. Total 57 bacterial isolates were collected from the 32 samples. Out of which, 33 LAB isolates were confirmed on the basis of morphological and preliminary biochemical characterization. On the basis of probiotic traits such as low pH and high bile salt tolerance, 8 LAB isolates with probiotic potentials were selected. These were further evaluated for phytase activity which is very important for availing various minerals and proteins in the gut. Two LAB isolates found to show significant phytase activity. Considering the probiotic potential along with phytase activity these isolates show health prospects and therefore they were characterized for identification. Morphological, biochemical and molecular characterization studies revealed Isolate 21 as strain of Pediococcus acidilactici (KU173544) and Isolate 24 (KU173545) as a strain of Pediococcus lolii.

Keywords: LAB, Bile salt, Probiotics, Phytase and 16 S rRNA.

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How to cite this article:

Dhingani, R.M., B.H. Joshi and Prasad, R.V. 2017. Screening, Characterization and Identification of Probiotic Potential LAB with Phytase ActivityInt.J.Curr.Microbiol.App.Sci. 6(9): 3539-3545. doi:
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