National Academy of Agricultural Sciences (NAAS)
 |
PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study evaluated the antagonistic effect of the rhizospheric chitinolytic bacterial isolates of rice and green gram soil samples against three phytopathogenic fungi, i.e. Pythium aphanidermatum (ITCC No.-5488), Fusarium oxysporum (ITCC No.-4998) and Rhizoctonia solani (ITCC No.-2060). A total of 25 bacterial isolates were obtained based upon their distinct colony characteristics on colloidal chitin agar medium. Only 6 isolates (G1, G2, R3, R4, R9 and R10) showed visible antagonistic activity against the test fungi by dual culture method on potato dextrose agar (PDA) during primary screening. During further screening by well diffusion method, all the 6 showed broad-spectrum antibacterial activity by inhibiting most of the test fungal phytopathogens. The six effective isolates were also able to reduce the fungal biomass of all the three test pathogens by mycelial reduction test. Though R10 inhibited all the three fungal pathogens but maximum reduction was observed against Rhizoctonia solani. Physiological & biochemical characterization of the effective isolates indicated that the antagonistic chitinolytic bacterial belong to Bacillus sp.
 |
 |
 |
 |
 |
