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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3358-3366
DOI: https://doi.org/10.20546/ijcmas.2017.609.414


Qualitative and Quantitative Analysis of Organic Acid Production Influenced by Phosphate Sources under Submerged Culture of Aspergillus niger a Phosphate Solubilising Fungi
Hruda Ranjan Sahoo and Nibha Gupta*
Division of Plant Pathology and Microbiology, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India
*Corresponding author
Abstract:

Aspergillus niger inoculated into medium containing Tricalcium phosphate (TCP) and Rock phosphate (RP) resulted in acid production which is indicated from the measure of pH and titrable acidity. Maximum of 105 mM acid production is recorded in TCP supplemented culture of 12 days whereas it is 124 mM in nutrient medium aided with RP and 14 days old culture. At the same time, decline of pH has also been observed in media containing TCP and RP. However, fungal biomass did not show any impact of both the kind of P sources. Chromatographic analysis of culture filtrate has showed the presence of citric and oxalic acid in TCP and RP respectively. Cultural conditions of different range of pH and incubation temperature do not play an important role in organic acid secretion by the fungi but organic acid secretion is mostly influenced by the nutritional sources such as carbon and nitrogenous compounds present in the medium.


Keywords: Aspergillus niger, Tricalcium Phosphate, Rock Phosphate, Organic acid.

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How to cite this article:

Hruda Ranjan Sahoo and Nibha Gupta. 2017. Qualitative and Quantitative Analysis of Organic Acid Production Influenced by Phosphate Sources under Submerged Culture of Aspergillus niger a Phosphate Solubilising FungiInt.J.Curr.Microbiol.App.Sci. 6(9): 3358-3366. doi: https://doi.org/10.20546/ijcmas.2017.609.414
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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