International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3149-3162

Phenotypic and Genotypic Analysis of Summer Mungbean Rhizobia
Moumita Ghosh1, Poonam Sharma2, Asmita Sirari2, Navprabhjot Kaur1 and Kailash Chand1*
1Department of Microbiology, Punjab Agricultural University, Ludhiana (141004), Punjab, India
2Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*Corresponding author

Phenotypic and genotypic diversity of rhizobia isolated from root and soil rhizosphere of summer mungbean was assessed on the basis of plant growth promoting traits and using RAPD primers. In present studies, isolates of rhizobia from nodules were purified on Congo red Yeast Mannitol Agar (CRYEMA) medium from summer mungbean growing areas of Punjab state, India. Slow and fast growing colonies of rhizobial isolates were selected on Bromo Thymol Blue (BTB) supplemented with YEMA medium. Among five RAPD primers used in present study, two of the primers RAPD-4 and RAPD-5 showed maximum number of detectable bands. Highest polymorphism detected with RAPD-5 during PCR amplification. Cluster analysis divided rhizobial isolates into five distinct groups. The data depicts that although the growth promoting parameter i.e. Indole acetic acid (IAA) production, phosphate solubilization were very promising in LSMR-1, LSMR-5, LSMR-8, LSMR-9, LSMR-19 and LSMR-23 but they were genetically diverse and fall under different groups of cluster analysis. Whereas, LSMR-1 and LSMR-5 showed a very close relation isolated from same native rhizosphere. The assessment of genetic diversity is a prerequisite and important step for the improvement of any legume crop. It is concluded that significant genetic diversity in rhizobial strains exists with respect to their efficiency for IAA production and P-solubilization.

Keywords: Bradyrhizobium sp., Indole acetic acid (IAA), P-solubilization, RAPD, Rhizobium sp., summer mungbean, UPGMA software

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Moumita Ghosh, Poonam Sharma, Asmita Sirari, Navprabhjot Kaur and Kailash Chand. 2017. Phenotypic and Genotypic Analysis of Summer Mungbean RhizobiaInt.J.Curr.Microbiol.App.Sci. 6(9): 3149-3162. doi:
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