National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The protein bands in ostrich seminal plasma (OSP) were identified by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and compared with NCBI reference sequence. The results revealed that OSP had five major specific proteins bands namely, OSP-I (GATA Zinc finger domain containing protein 1), OSP-II (GATA Zinc finger domain containing protein 1), OSP- III (E3 ubiquitin protein ligase RNF 216, OSP-IV (Mitotic spindle assembly checkpoint protein MAD1) and OSP-V (Dual specificity phosphatase DUPD1). The estimated molecular weight of OSP-I (Mr94.51 to 102.34 kDa), OSP-II (Mr75.19 to 93.07 kDa), OSP-III (Mr59.58 to 72.76 kDa), OSP-IV (Mr30.71 to 40.90 kDa) and OSP-V (Mr21.66 to 26.26 kDa). The results of our study provide basic knowledge of the protein composition of ostrich seminal plasma highlighting important physiological pathways which may play crucial roles in the sperm environment after ejaculation. This knowledge can be the basis to further develop procedures improving the reproduction of farmed ostrich.
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