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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3044-3049
DOI: https://doi.org/10.20546/ijcmas.2017.609.374


Post-Thaw Evaluation of Cryopreserved Boer Crossbred Buck Semen Extended in TEYG (Universal) Extender and its Fertility Rate
S. Manivannan, M. Selvaraju, R. Ezakial Napolean and P.S.L. Sesh
Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Namakkal, Tamil Nadu, India
*Corresponding author
Abstract:

Two Boer crossbred bucks aged between 41/2 and 5 years which are maintained at Frozen Semen Bank, Department of Veterinary Gynaecology and Obstetrics, Veterinary College and Research Institute, Namakkal were utilized for this study. The semen was collected from two adult Boer crossbred bucks. The collected ejaculates were immediately transferred to semen processing laboratory and kept at 34°C water bath. The semen collected from the two bucks were pooled in to a sterile glass tubes and immediately subjected to further processing and cryopreservation and finally stored in liquid nitrogen container. These semen straws were subjected evaluation by CASA at various storage periods. The sperm motility parameters decreased slowly as the duration increases. A total of 10 Tellicherry does were selected and all the does were inseminated with frozen thawed buck semen prepared from TRIS universal diluent and the pregnancy was confirmed by ultrasonography at 45 days of gestation. The fertility rate was 70 per cent using semen diluted with TEYG universal diluent. The survival rate and fertility rate was higher in cryopreserved Boer crossbred buck semen diluted with TEYG extender.


Keywords: Post-Thaw evaluation, TEYG, Fertility rate.

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How to cite this article:

Manivannan, S., M. Selvaraju, R. Ezakial Napolean and Sesh, P.S.L. 2017. Post-Thaw Evaluation of Cryopreserved Boer Crossbred Buck Semen Extended in TEYG (Universal) Extender and its Fertility RateInt.J.Curr.Microbiol.App.Sci. 6(9): 3044-3049. doi: https://doi.org/10.20546/ijcmas.2017.609.374
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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