National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Adhesion to intestinal epithelial cells is commonly analyzed during investigations on potential probiotic activity of certain bacteria. The aim of the present study was to investigate whether different prebiotics affect the adhesion properties of three strains lactobacilli (Lactobacillus rhamnosus 1010, Lactobacillus acidophilus11, and Lactobacillus paracasei 8458). Using in vitro model system based on cell lines derived from colon epithelial cells, we have determined the adherence ability of the three bacterial strains following treatment with 6 different prebiotics (inulin, pectin, chitosan, galacto-oligosaccharides, xylooligosaccharides and beta-glucan). Our results demonstrated significant reduction of lactobacilli adhesion after treatment with beta-glucan and chitosan. Conversely, xylooligosaccharides- and galacto-oligosaccharides-treated bacteria showed enhanced ability to adhere to enterocyte-like cell lines. Treatment with inulin did not show pronounced effect on lactobacilli adhesion. Overall, the three tested Lactobacillus strains displayed similar responses to the prebiotic treatment. Only pectin induced strain specific effect – reduced adhesion of L. rhamnosus 1010 and L. acidophilus 11 while the adhesion properties of L. paracasei 8458 were not significantly affected. These findings suggest that different types of prebiotic substances induce different mechanisms that modulate the adhesion ability and probably the probiotic activity of lactobacilli.
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