National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Myostatin or growth and differentiation factor 8 (GDF8), is a skeletal muscle regulator factor that determines muscle mass in animals including human beings. Mutation in the myostatin gene sequence controls its regulating function and results in growth via hypertrophy of muscles. Mutation in myostatin gene has been found in various species of animals and has three exons and two introns in all species. Considering the MSTN gene as a candidate gene for gene assisted selection for growth traits, the present study was aimed to study its polymorphism and its association in Boer and Bakerwal goats in Kashmir valley. We screened polymorphism of the myostatin gene in 600 goats from two goat populations (Boer and Bakerwal) and applied PCR and DNA sequencing analysis to reveal single nucleotide polymorphisms (g.368A>C (p.Lys to Thr). At g.368A>C locus, the frequencies of g.368A allele were 0.70–0.80, and the frequencies of g.368C allele were 0.20–0.30 in Boer goats. And at g.368A>C locus, the frequencies of g.368A allele were 0.60–0.80, and the frequencies of g.368C allele were 0.20–0.40 in Bakerwal goats. Compared to AC genotype, those with AA genotype had superior body weight in Boer goats (12 & 14 months) (41.68 ±0.41 vs 43.21 ±0.31, P<0.05) (43.57 ±0.41 vs 46.00 ±0.05, P<0.05) respectively. In addition, the Boer goats (13 months) with AA genotype (72.50 ±0.50 cm) had greater body height than those with AC genotype (70.37 ±0.26cm). Similarly, AA genotype in Bakerwal goats have high body weight than AC genotype (40.16 ±0.54 vs 42.75 ±0.25), P<0.01 (12 months), body length (81.12 ±0.63 vs 86.00 ±1.00), P<0.05 (14 months) and chest circumference (86.42 ±0.61 vs 89.66 ± 0.33), P<0.05 (13 months) respectively. These SNPs would provide molecular genetic material for studying the meat performance of goats.
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