International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 2572-2578

Compatibility of Trichoderma harzianum (KBN-29) with Selected Inorganic Fertilizers: An In vitro Study
Kishor Chand Kumhar1*, A. Babu1, John Peter2, M. Bordoloi1, Hirakjyoti Rajbongshi1, Sarvesh Singh Yadav1, Swagata Mani1 and Pritam Dey1
1Tea Research Association, North Bengal Regional Research and Development Centre,
Nagrakata, District - Jalpaiguri, West Bengal 735 225, India
2Varsha Bioscience and Technology India Pvt. Ltd. # 253/A, Jiblakpally Village, Pochampally Mandal, Nalgonda, Hyderabad - 508284, Telangana, India
*Corresponding author

The compatibility of indigenous Trichoderma harzianum (KBN-29) with a few foliar fertilizers i.e. muriate of potash (MoP), urea and zinc sulphate was studied under in vitro conditions. The recommended doses of fertilizers (1 and 2% w/v) were tested using solid (PDA) as well as liquid (PDB) media. When these fertilizers were tested individually on solid medium, the antagonist could produce 8.5, 6.9 and 2.6 cm diameter colony in presence of MoP, urea and zinc sulphate, respectively, which indicated, that zinc sulphate as the least compatible when compared to fertilizers. Similarly, in liquid medium, the antagonist could produce 0.76, 0.63 and 0.53 g biomass as compared to untreated control (0.85 g) that showed the similar trend. When the combination of all three fertilizers was tested in solid medium, T. harzianum could develop the colony of 1.4 to 3.4 cm diameter which is significantly less as compared to control (8.7 cm). In case of liquid medium the combination of fertilizers exhibited the similar pattern where the antagonist yielded lesser biomass (0.20 to 0.21 g) compared to control (0.89 g). The study revealed that MoP and urea are compatible with the antagonist, however zinc sulphate was found to be the least compatible.

Keywords: Trichoderma harzianum, Inorganic fertilizer, Compatibility, Vegetative growth, Sporulation.

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Kishor Chand Kumhar, A. Babu, John Peter, M. Bordoloi, Hirakjyoti Rajbongshi, Sarvesh Singh Yadav, Swagata Mani and Pritam Dey. 2017. Compatibility of Trichoderma harzianum (KBN-29) with Selected Inorganic Fertilizers: An In vitro StudyInt.J.Curr.Microbiol.App.Sci. 6(9): 2572-2578. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.