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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study document the isolation and enumeration of siderophore producing microbes from iron contaminated soil. Identification of potential strains and optimization studies for the production of siderophore was carried out. The Iron contaminated soil samples were collected from welding shops, factories in and around Kanchipuram and Thiruvallur. Ten morphologically different strains (AS1-AS10) were selected for further screening for the production of siderophores by Ferric Perchlorate Assay. Strain AS-6 showed a maximum OD value of 0.82 and was further identified as Pseudomonas sp and optimization studies were carried out. Siderophore production was maximum when using Glucose medium, 72 hours of incubation; pH 5.5, 300C; Ferric Chloride as additive. The partially purified siderophore showed good zone of inhibition against water borne pathogens such as Salmonella, Vibrio, Shigella, and E.coli.