International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 1-9

First Detection of TEM-116 and SHV-75 Producing Enterobacteria Isolated from Two Ivorian eaching Hospitals: Case of Abidjan and Bouake
A.A. Toty1,2*, N. Guessennd2, C. Akoua-Koffi3, D. A. Otokore1, C. Meex4, G.V. Mbengue2, A. J. Djaman1, M. Dosso2 and M. Galleni5
1Laboratoire de Pharmacodynamie Biochimique, UFR Biosciences, Universite Felix Houphouet Boigny d’Abidjan, 22 BP 582 Abidjan 22 Côte d’Ivoire
2Laboratoire de Bacteriologie-Virologie, Unite des Antibiotiques, des Substances Naturelles et de la Surveillance des Resistances des Micro-Organismes aux Anti-Infectieux (ASSURMI) de l’Institut Pasteur de Cote d’Ivoire, 08 BP 1563 Abidjan 08
3Laboratoire de Bacteriologie, Centre Hospitalier Universitaire de Bouake, Cote d’Ivoire
4Service de Microbiologie Clinique, CHU Start Tilman, B-4000 Liege, Belgique
5Centre d’Ingenierie des Proteines, Universite de Liege, Institut de Chimie B6a, Allee du 6 Aout, 11 Start Tilman-B4000, Liege, Belgique
*Corresponding author

The aim of this study was to detect and identify blaTEM, blaSHV and blaOXA genes in Abidjan and Bouaké. A total of 73 strains of Enterobacteriaceae from Abidjan and Bouaké and resistant to at least two third generation cephalosporins have been taken into account. Maldi-Tof and Vitek-2, double-disc synergy method were used for identification, determination of minimum inhibitory concentrations (MIC) and the ESBL detection. The blaTEM, blaSHV and blaOXA genes were determined by PCR and the β-lactamases identified by sequencing. The overall prevalence of 56.2% was observed with rates of 65.8% for K. pneumoniae, 24.4% for E. coli, 7.3% for E. cloacae, 2.4% M. morganii and 0% in P. mirabilis. The blaTEM, blaSHV, blaOXA genes were detected at respective rates of 90.2%, 87.8% and 12.2%. The identification revealed the presence of TEM-1, TEM-116, SHV-11, SHV-12, SHV-75 and OXA-1. The strains were resistant to ceftazidime and cefotaxime (100%), to cefepime (95%) and susceptible to ertapenem and meropenem (100%). Our study showed an increase in the prevalence of TEM and OXA ESBLs and the first detection of TEM-116 and SHV-75 in Côte d’Ivoire.

Keywords: Enterobacteria,β-lactamases, blaTEM-116, blaSHV-75,Cote d’Ivoire

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How to cite this article:

Toty, A.A., N. Guessennd, C. Akoua-Koffi, D.A. Otokore, C. Meex, G.V. Mbengue, A.J. Djaman, M. Dosso and Galleni, M. 2016. First Detection of TEM-116 and SHV-75 Producing Enterobacteria Isolated from Two Ivorian Teaching Hospitals: Case of Abidjan and Bouake.Int.J.Curr.Microbiol.App.Sci. 5(5): 1-9. doi:
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