National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study was to detect and identify blaTEM, blaSHV and blaOXA genes in Abidjan and Bouaké. A total of 73 strains of Enterobacteriaceae from Abidjan and Bouaké and resistant to at least two third generation cephalosporins have been taken into account. Maldi-Tof and Vitek-2, double-disc synergy method were used for identification, determination of minimum inhibitory concentrations (MIC) and the ESBL detection. The blaTEM, blaSHV and blaOXA genes were determined by PCR and the β-lactamases identified by sequencing. The overall prevalence of 56.2% was observed with rates of 65.8% for K. pneumoniae, 24.4% for E. coli, 7.3% for E. cloacae, 2.4% M. morganii and 0% in P. mirabilis. The blaTEM, blaSHV, blaOXA genes were detected at respective rates of 90.2%, 87.8% and 12.2%. The identification revealed the presence of TEM-1, TEM-116, SHV-11, SHV-12, SHV-75 and OXA-1. The strains were resistant to ceftazidime and cefotaxime (100%), to cefepime (95%) and susceptible to ertapenem and meropenem (100%). Our study showed an increase in the prevalence of TEM and OXA ESBLs and the first detection of TEM-116 and SHV-75 in Côte d’Ivoire.
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