National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Forced and unforced shoot tips were surface sterilized with different sterilant regimes and incubated under normal culture room conditions. Satisfactory culture asepsis (55.99%) and explant survival (56.66%) was achieved in forced explants by subjecting them to the treatment of 0.1 per cent Mercuric chloride for 10 minutes followed by 70 per cent ethyl alcohol for 10 seconds. Murashige and Skoog’s full strength medium with 0.50 mg /l BAP and 0.01 mg/l IBA gave the best response in terms of establishment of cultures. Murashige and Skoog’s full strength basal media supplemented with BAP and IBA at different concentrations were tried for culture proliferation of quince and it was found that BAP at 0.50 mg/l and IBA at 0.01 mg/l gave the highest per cent proliferated cultures (72.49 %), Shoot number/explants(5.75cm) and maximum shoot length(5.49cm). However, the lowest percentage of proliferated cultures (41.66%) were observed in WPM medium supplemented with BAP 0.50mg/l and IBA 0.01mg/l. Microcuttings (10-15mm) from the proliferated cultures were inoculated in two media supplemented with different IBA concentrations for rooting.MS medium fortified with IBA (1.25mg/l) resulted in maximum rooting (81.66%), root number/shoot (5.00%) and root length (6.15cm).
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