International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 784-792

Screening and Partial Purification of L-Asparaginase from the Bacteria Isolated from Soil Samples
A.V. Savani1 and P.R. Parmar2*
1C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University,Tarsadi, Surat – 394 350, Gujarat, India
2Main Cotton Research Station, Navsari Agricultural University, Surat – 395 007, Gujarat, India
*Corresponding author

L-asparaginase producing bacteria were isolated from the selected soil samples from different location of Surat district, Gujarat. The primary screening using rapid plate assay determined that out of 120 isolates, 33 strains were producing L-asparaginase. Among 33 L-Asparaginase Producing Bacterial strains (APB), significant pink color zone were observed in APB2 (0.8 mm), APB1 (0.9 mm), APB28 (0.9 mm) and APB26 (1.2 mm) at 72 hrs. Further, these four isolates preliminary screened for fermentation at laboratory level by observing pH, biomass and crude enzyme activity. Among four isolates, APB26 produced substantial L-asparaginase (3.78 unit/ml) followed by APB1 (1.58 unit/ml), APB2 (1.08 unit/ml) and APB28 (0.80 unit/ml) within 72 hrs. Further, the attempt was made to optimize pH and temperature on L-asparaginase production by APB26 within 72 hrs. The results showed that pH 7.0 and 37oC temperature was optimum. Partial purification with ammonium sulfate precipitation showed 2.59 units/ml specific activity with 89.3% yield and 1.16 purification fold while, DEAE cellulose chromatography given 3.57 unit/ml specific activity with 66.3% yield and 1.60 purification fold. Based on morphological, cultural and biochemical test, APB26 was tentatively identified as Bacillus species.

Keywords: Isolation, L-asparaginase, Partial purification, Soil samples, Bacillus.

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How to cite this article:

Savani, A.V. and Parmar, P.R. 2017. Screening and Partial Purification of L-Asparaginase from the Bacteria Isolated from Soil Samples.Int.J.Curr.Microbiol.App.Sci. 6(9): 784-792. doi:
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