National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Vaccination is one of the best preventive measures against viral diseases in aquaculture. In this study, experimental inactivated similar damselfish virus (SRDV) vaccine was prepared and immunogenicity and protection against virus infection of the vaccine was investigated in koi carp (Cyprinus carpio). Formalin-inactivated virus at 4°C for 2 days was exhibited no typical clinical symptoms of SRDV infection and no mortality following challenge at 28-day post-vaccination. The immune response was induced by intraperitoneal injection of koi with formalin-inactivated vaccine added Quil-A® adjuvant. The expression levels of genes including IRF-7 and IL-10 in spleen of koi immunized with vaccine added Quil-A® adjuvant was highly up-regulated (52.2 fold) at 24 h post infection of the virus but in kidney was showed down-regulation (0.4 fold). Interestingly, the highest level of up-regulation (16 fold) was recorded at 96 h post infection of SRDV. Like IRF-7, highest up-regulation of IL-10 gene was observed in spleen of koi immunized with vaccine added Quil-A® adjuvant at 24 post infection of the virus while kidney was showed down-regulation. The study provides strong evidence for the existence of expression profiles of immune related genes (IRF-7 and IL-10) during the ranavirus infection. It also suggests that Quil-A® adjuvant enhances the immune response of the vaccine candidates.
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