National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The environmental pollution and risk of resistance development in the pathogen by the use of chemical pesticide makes necessary to use biological agents. However the major limitations to utilize biological agents are the inconsistency, unreliable efficacy and short shelf life of the living entities used in the formulation. In this context an experiment was conducted to formulate four bacterial bio-agents viz., Bacillus subtilis-KK-9A, Brevibacillus borstelensis-BK-6, Brevibacillus sp-PM-2A and Lysinibacillus xylanilyticus- VK-6B and consortium of them. Seven carrier materials viz., bentonate, kaolin, perlite, phytosil, talc, vermiculite and zeolite were used and studied the shelf life of bio-agents in this carrier material up to 90 days. Among seven carrier materials vermiculite recorded the highest number of colonies of all the four bio-agents and consortium when checked at fifteen days interval up to three months. This is followed by zeolite, kaolin, perlite, talc, bentonite and phytosil in the order of their efficacy. Further the highest survival % (21.6, 46.3, 30.3, 28.2 and 32.1) of all the four bio-agents and consortium respectively was observed in vermiculite based formulation. However, least survival percentage of Bacillus subtilis-KK-9A (3.7), Brevibacillus borstelensis-BK-6(3.15), Brevibacillus sp-PM-2A (3.7) and Lysinibacillus xylanilyticus- VK-6B (10.1) and consortium (2.9) was recorded in phytosil based formulation at 90th day.
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