International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:9, September, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1098-1102

Detection and Characterization of Infectious Bronchitis Virus in Desi Birds by Molecular Assay
A.P. Surendar*, K.M. Palanivel, S. Saravanan and R. Gopala Krishnamurthy
Department of veterinary preventive medicine, Veterinary College and Research Institute, Namakkal-2, Tamil Nadu Veterinary and Animal Sciences University, India
*Corresponding author

Infectious Bronchitis (IB) is highly contagious and acute, with economically importance disease of poultry. 185 samples including tracheal swab and faecal swab were collected from apparently healthy and infected birds with respiratory disorders. An attempt made to isolate the virus through inoculation of specific pathogen free embryonated egg (SPFEE) as cultural medium. The embryo showing dwarfism were identified and subjected to molecular diagnosis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify N-Protein and sequenced. Factors responsible for prevalence of IB in desi chicken were studied. Out of 185 samples 7 samples showed dwarfism in SPFEE. 4 out of 7 samples, found positive for IBV by RT-PCR.

Keywords: Infectious Bronchitis, Isolation, Detection, RT-PCR, NP glycoprotein.

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How to cite this article:

Surendar, A.P., K.M. Palanivel, S. Saravanan and Gopala Krishnamurthy, R. 2017. Detection and Characterization of Infectious Bronchitis Virus in Desi Birds by Molecular Assay.Int.J.Curr.Microbiol.App.Sci. 6(9): 1098-1102. doi:
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