National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacillus thuringiensis produces different insecticidal crystal proteins which are encoded by cry genes. Isolation was carried out from 17 diverse locations’ soil; nine bacterial isolates were separated and selected on the basis of presence of parasporal body. Isolate ABt 10 was screened for different cry genes with universal and gene specific primers. Results indicated the presence of cry1Ac and cry1Ab, cry 4, and cry 1D genes in native isolate ABt 10. All these clearly indicated the specificity of these isolates for Lepidopteran insect pests. The search and selection of native isolates will be useful in the generation of a transgenic plant with an indigenous cry gene that offers resistance to the serious local pests.
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