National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Endophytic bacteria residing within the living plant tissue without causing harm to it have promise to be practically used in agriculture and included as active components of Integrated Disease Management (IDM). In present study 4 strains of pseudomonad were isolated from surface sterilized plant parts viz. leaf, root, tuber and stem of potato (Solanum tuberosum L. cv. Kufri Badshah) by using King’s B agar and preliminary characterized as Pseudomonas on the basis of morphological and biochemical characteristics. Among all the isolates KPSE3 was emerged as best PGP strain showing production of siderophore and IAA (9.79 μg/ml). Moreover, KPSE3 has capacity of nitrogen fixation (23.57 mg N / g glucose consumed) and phosphate solubilization (21.20 P μg/ml at 3 DAI). The isolate KPSE3 also have broad spectrum antifungal activity with 15 % growth inhibition of Pythium sp. 34% of Fusarium sp. and 40 % of Macrophomina sp. In pot trial conditions potato tuber bacterization with KPSE3 in presence of phytopathogens viz. Fusarium sp., Macrophomina sp. and pythium sp., significantly influenced growth of Potato cv. Kufri Badshah. The endophytic isolate KPSE3 exhibiting the best PGP activity was further characterized at molecular level and identified as Pseudomonas aeruginosa (Gene Bank Accessions Number- KC787580) which can be used for developing a cost - effective eco-friendly multifunctional biofertilizer and biocontrol agent for sustainable agriculture.
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