National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Probiotic delivery through feed is a practical route for introducing beneficial microorganisms into aquaculture systems, but processing and storage conditions can influence bacterial survival. The present study evaluated the preparation of a post-pellet probiotic feed using the fish gut-derived Lactiplantibacillus plantarum, and examined growth-related responses during a controlled feeding trial. An actively growing culture was harvested, washed and concentrated before being mixed with a binder and applied to cooled feed pellets. Control and probiotic-coated diets were prepared under aseptic conditions and stored under refrigeration before use. Fish were acclimatized and allocated to control and probiotic-feed groups maintained under comparable tank conditions for the experimental period. Water quality remained within the ranges recorded in the thesis: temperature 27.1–27.3 °C, pH 7.2–7.3, dissolved oxygen 5.8–6.0 mg/L, salinity approximately 12 ppt and ammonia 0.014–0.015 mg/L. Initial body weight was 10 g in both groups, while the recorded final weights were 22 g in the control and 28 g in the probiotic group. The higher specific growth rate was reported for probiotic-fed fish than for controls and lower feed conversion ratio (17.86 versus 25.00). The study nevertheless demonstrates the feasibility of post-pelleting incorporation of the selected Lactiplantibacillus plantarum and provides preliminary evidence of a favourable growth response under the recorded experimental conditions.
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