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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles Volume : Specialissue, Issue : 13, January,

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci..Specialissue(13) : 148-158
DOI : https://doi.org/10.20546/ijcmas.2026.sp13.017


Evaluation of Herbal Mosquito Repellent Formulations Prepared from Ocimumtenuiflorum and Coleus amboinicus Leaf Extracts

Department of Microbiology, Chennai National Arts and Science College, Avadi, Chennai – 54, Tamil Nadu, India
*Corresponding author
Abstract:

Mosquitoes are important vectors of several infectious diseases, creating a need for effective plant-based approaches for personal protection. The present study evaluated the phyto-chemical characteristics and mosquito-repellent potential of aqueous leaf extracts of Ocimumtenuiflorum and Coleus amboinicus, individually and in combination. Fresh leaves were extracted with distilled water at a 1:10 (w/v) ratio and subjected to qualitative phytochemical screening. Tulasi extract showed positive reactions for tannins and saponins, whereas Karpooravalli extract showed terpenoids, glycosides and flavonoids. The extracts were incorporated into a vaseline base and evaluated using a screened-cage assay with 20 adult mosquitoes for 2 h. Tulasi treatment resulted in 5 deaths, while Karpooravalli resulted in 8 deaths. The 1:1 combination produced 10 deaths and was associated with pronounced avoidance and reduced landing behaviour. The formulated preparation showed a smooth and uniform appearance without visible phase separation during the observation period. The findings indicate that O. tenuiflorum and C. amboinicus extracts possess observable mosquito-deterrent activity and that their combined formulation warrants further investigation using standardized quantitative repellency assays, replicated experiments and safety evaluation.


Keywords: Ocimumtenuiflorum; Coleus amboinicus; mosquito; herbal formulation; phytochemical screening; aqueous extract


References:

Amer, A., and Mehlhorn, H. (2006). Repellency effect of forty-one essential oils against Aedes, Anopheles and Culex mosquitoes. Parasitology Research, 99(4), 478–490. https://doi.org/10.1007/s00436-006-0184-1.

Bairwa, G. P., Sharma, M., Ahuja, A., Tiwari, S., & Devi, J. (2020). Formulation and evaluation of herbal ointment containing extracts of medicinal plants. International Journal of Pharmaceutical Sciences Review and Research, 64(1), 45–49.

Fradin, M.S., and Day, J.F. (2002). Comparative efficacy of insect repellents against mosquito bites. New England Journal of Medicine, 347(1), 13–18. https://doi.org/10.1056/NEJMoa011699.

Ghosh, A., Chowdhury, N., & Chandra, G. (2012). Plant extracts as potential mosquito larvicides. Indian journal of medical research, 135(5), 581-598.

Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. Chapman & Hall, London.

Kamaraj, C., Bagavan, A., Elango, G., Zahir, A., Rajakumar, G., Marimuthu, S., Santhoshkumar, T., & Rahuman, A. (2011). Larvicidal activity of medicinal plant extracts against Anopheles subpictus & Culex tritaeniorhynchus. Indian Journal of Medical Research, 134(1), 101–106.

Kumar, M., & Kamal, B. S. (2021). Promising mosquito repellent and mosquito larvicidal plants from the genus Coleus: A review. International Journal of Botany Studies, 6(6), 479–484. https://www.botanyjournals.com/archives/2021/vol6/issue6

Maia, M.F., and Moore, S.J. (2011). Plant-based insect repellents: a review of their efficacy, development and testing. Malaria Journal, 10(Suppl 1), S11. https://doi.org/10.1186/1475-2875-10-S1-S11.

Megha Rani N., Sridevi K., & Rao S.N. (2016). Preliminary phytochemical analysis of fresh juice and aqueous extract of Coleus amboinicus Linn leaves. International Journal of Applied Biology and Pharmaceutical Technology, 7(1), 1–5.

Ragavendran, K., Selvakumaran, J., Muthukanagavel, M., Alharbi, N.S., Thiruvengadam, M., Mutheeswaran, S., Ignacimuthu, S., and Ganesan, P. (2024). Chemical composition and mosquitocidal properties of essential oil from Indian indigenous plants Ocimumtenuiflorum L. and Ocimumamericanum L. against three vector mosquitoes. Experimental Parasitology, 258, 108709. https://doi.org/10.1016/j.exppara.2024.108709.

Reinhold, J.M., Lazzari, C.R., & Lahondère, C. (2018). Effects of environmental temperature on Aedesaegypti and Aedesalbopictus mosquitoes: Implications for dengue and chikungunya transmission. Parasites & Vectors, 11(1), 738.

Sharma A, Manisha Dikshit, AC Pandey, Raj Kumar Singh, Praful Dikshit (2023). Larvicidal activity of essential oils of Haridra and Tulsi against Aedesaegypti and Anopheles larvae. J Ayurveda Integr Med Sci [Internet]. 2023 Jun. 25 [cited 2026 Sep. 23]; 8(5): 13-20. Available from: https://jaims.in/jaims/article/view/2383

Sneha K, Narayanankutty A, Job JT, Olatunji OJ, Alfarhan A, Famurewa AC, Ramesh V. (2022). Antimicrobial and Larvicidal Activities of Different Ocimum Essential Oils Extracted by Ultrasound-Assisted Hydrodistillation. Molecules. 2022 Feb 22; 27(5): 1456. https://doi.org/10.3390/molecules27051456. PMID: 35268557; PMCID: PMC8911667.

Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. 2nd ed. Spectrum Books Ltd., Ibadan.

Vaibhavi Savalia, Ishita Zalavadiya, Kaushal Vala, Pavan Badhai (2026). Essential and Fixed Oils from Plants: A Natural Approach to Mosquito Management. EPJ Web Conf. 348 01028 https://doi.org/10.1051/epjconf/202634801028

World Health Organization (2009). Guidelines for efficacy testing of mosquito repellents for human skin. WHO/HTM/NTD/WHOPES/2009.4. Geneva: World Health Organization.

World Health Organization (WHO). (2013). Guidelines for efficacy testing of spatial repellents. Geneva: World Health Organization. ISBN 978 92 4 150502 4.


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How to cite this article:

Sangeetha M. and Veppalai Balagangatharam Malathi. 2026. Evaluation of Herbal Mosquito Repellent Formulations Prepared from Ocimumtenuiflorum and Coleus amboinicus Leaf Extracts Int.J.Curr.Microbiol.App.Sci. Specialissue(13): 148-158 doi: https://doi.org/10.20546/ijcmas.2026.sp13.017
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license

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