National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Klebsiella pneumoniae is an important opportunistic pathogen associated with bovine mastitis and may serve as a reservoir of antimicrobial resistance determinants within dairy production systems. The present study investigated the occurrence of K. pneumoniae and characterized extended-spectrum β-lactamase (ESBL) production and multidrug resistance among isolates recovered from bovine mastitis and dairy farm-associated samples. A total of 584 samples were collected from 30 dairy farms, comprising 494 milk samples and 90 farm-associated samples, including hand swabs, Cowshed swabs and sewage. Bacterial isolates were identified by cultural, morphological and biochemical characteristics and confirmed by 16S rRNA gene amplification and sequencing. Antimicrobial susceptibility was determined by the Kirby–Bauer disc diffusion method according to CLSI guidelines. ESBL production was evaluated phenotypically by the double-disc synergy test (DDST) and MIC-based E-test. ESBL-associated blaCTX-M, blaSHV and blaTEM genes were detected by PCR. Twenty K. pneumoniae isolates were recovered, of which 12 (60.0%) originated from mastitic milk and eight (40.0%) from farm-associated samples. Eight isolates (40.0%) were resistant to third-generation cephalosporins and seven (35.0%) were confirmed as ESBL producers. All ESBL isolates carried blaCTX-M, while blaSHV and blaTEM were detected in three (42.9%) and two (28.6%) isolates, respectively. Thirteen isolates (65.0%) were multidrug resistant, including all seven ESBL producers. The findings demonstrate the occurrence of ESBL-producing MDR K. pneumoniae in bovine mastitis and associated dairy farm environments, highlighting the importance of antimicrobial surveillance and prudent antimicrobial use in dairy production systems.
Aarestrup, F.M. (2015). The livestock reservoir for antimicrobial resistance: a personal view on changing patterns of risks, effects of interventions and the way forward. Philosophical Transactions of the Royal Society B: Biological Sciences, 370: 20140085. https://doi.org/10.1098/rstb.2014.0085
Bandyopadhyay, S., Banerjee, J., Bhattacharyya, D., Samanta, I., Mahanti, A., Dutta, T.K., Ghosh, S., Nanda, P.K., Dandapat, P. and Bandyopadhyay, S. (2018). Genomic identity of fluoroquinolone-resistant blaCTX-M-15-type ESBL and pMAmpC β-lactamase producing Klebsiella pneumoniae from buffalo milk, India. Microbial Drug Resistance, 24: 1345–1353. https://doi.org/10.1089/mdr.2017.0368
Bradley, A.J. (2002). Bovine mastitis: an evolving disease. The Veterinary Journal, 164: 116–128. https://doi.org/10.1053/tvjl.2002.0724
Bush, K. and Bradford, P.A. (2016). β-Lactams and β-lactamase inhibitors: an overview. Cold Spring Harbor Perspectives in Medicine, 6: a025247. https://doi.org/10.1101/cshperspect.a025247
Cantón, R., González-Alba, J.M. and Galán, J.C. (2012). CTX-M enzymes: origin and diffusion. Frontiers in Microbiology, 3: 110. https://doi.org/10.3389/fmicb.2012.00110
Carattoli, A. (2009). Resistance plasmid families in Enterobacteriaceae. Antimicrobial Agents and Chemotherapy, 53: 2227–2238. https://doi.org/10.1128/AAC.01707-08
Clinical and Laboratory Standards Institute (CLSI). (2020). Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100. Wayne, Pennsylvania, USA: Clinical and Laboratory Standards Institute.
D’Andrea, M.M., Arena, F., Pallecchi, L. and Rossolini, G.M. (2013). CTX-M-type β-lactamases: a successful story of antibiotic resistance. International Journal of Medical Microbiology, 303: 305–317. https://doi.org/10.1016/j.ijmm.2013.02.008
Halasa, T., Huijps, K., Østerås, O. and Hogeveen, H. (2007). Economic effects of bovine mastitis and mastitis management: a review. Veterinary Quarterly, 29: 18–31. https://doi.org/10.1080/01652176.2007.9695224
Kar, D., Bandyopadhyay, S., Bhattacharyya, D., Samanta, I., Mahanti, A., Nanda, P.K., Mondal, B., Dandapat, P., Das, A.K., Dutta, T.K., Bandyopadhyay, S. and Singh, R.K. (2015). Molecular and phylogenetic characterization of multidrug resistant extended spectrum β-lactamase producing Escherichia coli isolated from poultry and cattle in Odisha, India. Infection, Genetics and Evolution, 29: 82–90. https://doi.org/10.1016/j.meegid.2014.11.003
Koovapra, S., Bandyopadhyay, S., Das, G., Bhattacharyya, D., Banerjee, J., Mahanti, A., Samanta, I., Nanda, P.K., Kumar, A., Mukherjee, R. and Dimri, U. (2016). Molecular signature of extended spectrum β-lactamase producing Klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern India. Infection, Genetics and Evolution, 44: 395–402. https://doi.org/10.1016/j.meegid.2016.07.032
Magiorakos, A.P., Srinivasan, A., Carey, R.B., Carmeli, Y., Falagas, M.E., Giske, C.G., Harbarth, S., Hindler, J.F., Kahlmeter, G., Olsson-Liljequist, B., Paterson, D.L., Rice, L.B., Stelling, J., Struelens, M.J., Vatopoulos, A. and Monnet, D.L. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18: 268–281. https://doi.org/10.1111/j.1469-0691.2011.03570.x
Markey, B., Leonard, F., Archambault, M., Cullinane, A. and Maguire, D. (2013). Clinical Veterinary Microbiology. 2nd ed. Mosby Elsevier, Edinburgh, UK.
Martínez, J.L., Coque, T.M. and Baquero, F. (2015). What is a resistance gene? Ranking risk in resistomes. Nature Reviews Microbiology, 13: 116–123. https://doi.org/10.1038/nrmicro3399
Munoz, M.A., Welcome, F.L., Schukken, Y.H. and Zadoks, R.N. (2007). Molecular epidemiology of two Klebsiella pneumoniae mastitis outbreaks on a dairy farm in New York State. Journal of Clinical Microbiology, 45: 3964–3971. https://doi.org/10.1128/JCM.00795-07
Ojdana, D., Sacha, P., Wieczorek, P., Czaban, S., Michalska, A., Jaworowska, J., Jurczak, A., Poniatowski, B. and Tryniszewska, E. (2014). The occurrence of blaCTX-M, blaSHV, and blaTEM genes in extended-spectrum β-lactamase-positive strains of Klebsiella pneumoniae, Escherichia coli and Proteus mirabilis in Poland. International Journal of Antibiotics, 2014: 935842. https://doi.org/10.1155/2014/935842
Paterson, D.L. and Bonomo, R.A. (2005). Extended-spectrum β-lactamases: a clinical update. Clinical Microbiology Reviews, 18: 657–686. https://doi.org/10.1128/CMR.18.4.657-686.2005
Ruegg, P.L. (2017). A 100-year review: mastitis detection, management, and prevention. Journal of Dairy Science, 100: 10381–10397. https://doi.org/10.3168/jds.2017-13023
Ruegg, P.L. and Reinemann, D.J. (2002). Milk quality and mastitis tests. The Bovine Practitioner, 36: 41–54. https://doi.org/10.21423/bovine-vol36no1p41-54
Snedecor, G.W. and Cochran, W.G. (1967). Statistical Methods. 6th ed. Iowa State University Press, Ames, Iowa, USA.
Wyres, K.L. and Holt, K.E. (2018). Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria. Current Opinion in Microbiology, 45: 131–139. https://doi.org/10.1016/j.mib.2018.04.004
Xu, T., Wu, X., Cao, H., Pei, T., Zhou, Y., Yang, Y. and Yang, Z. (2022). The characteristics of multilocus sequence typing, virulence genes and drug resistance of Klebsiella pneumoniae isolated from cattle in Northern Jiangsu, China. Animals, 12: 2627. https://doi.org/10.3390/ani12192627
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