National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Petroleum-based plastics persist in the environment, creating a need for biodegradable alternatives. This study investigated PHA production by a rhizosphere isolate identified as Bacillus subtilis and its incorporation into an agar–chitosan film. PHA production was screened using Mineral Davis Medium and Sudan Black B staining. The polymer was extracted using sodium hypochlorite–chloroform treatment and incorporated into the film with glycerol as a plasticizer. The film was evaluated by FTIR, solubility, melting-point, and soil-burial tests. PHA was insoluble in water but soluble in chloroform. The film showed a melting point of approximately 70°C and 26.9% weight loss after 20 days of soil burial. The findings support the potential of PHA-based films for biodegradable packaging applications.
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