National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Food-borne bacterial infections remain an important concern for public health and food preservation, creating interest in plant-derived antimicrobial formulations. This study evaluated the antibacterial performance of combined aqueous and ethanolic extracts prepared from six medicinal plants: Asparagus racemosus, Acacia arabica, Andrographis paniculata, Lawsonia inermis, Chrysanthemum odoratum, and Centella asiatica. Extract combinations containing two to six plant species were screened against Staphylococcus aureus, Bacillus cereus, Escherichia coli O157:H7, Salmonella enteritis, and Listeria monocytogenes using an agar disc-diffusion assay. Ciprofloxacin was included as the reference antibacterial agent. The results showed that antibacterial performance depended on the composition of the mixture and was not directly proportional to the number of plants combined. Among the evaluated formulations, a three-plant combination comprising C. odoratum, L. inermis, and A. paniculata displayed the most consistent broad-spectrum inhibition. The ethanolic preparation produced inhibition zones of 2.1–2.4 cm, whereas the corresponding aqueous preparation produced zones of 1.8–2.0 cm. The maximum inhibition was observed against S. enteritis. Larger combinations containing four to six plant species retained antibacterial activity but did not exceed the performance of the selected three-plant formulation. The findings indicate that carefully selected plant combinations may provide enhanced antibacterial effects and warrant further investigation using MIC, MBC, checkerboard interaction assays, phytochemical profiling, and food-model validation.
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