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Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56
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Volume-15, Issue-3 Published

Call for paper-Vol-15, Issue 5- May 2026

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Original Research Articles Volume : 15, Issue : 5, May, 2026

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

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Int.J.Curr.Microbiol.App.Sci.2026.15(5) : 70-76

DOI : https://doi.org/10.20546/ijcmas.2026.1505.010

Isolation, Characterization and Antimicrobial activity of Biosurfactant Produced by Yeast isolate YNWaG

Nahida Fatima^1,3, M. Yahya Khan² and B. S. Anuradha^3*

¹Department of Microbiology, Kasturba Gandhi Degree & PG College for Women, Sec’bad, Telangana 500026, India ²Kalam’s Institute of Science, Nallakunta, Hyderabad, Telangana 500044, India ³Department of Microbiology, Chaitanya (Deemed to be University), Hanamkonda, Telangana 506001, India

^*Corresponding author

Abstract:

Biosurfactants are extracellular secondary metabolites produced by microorganisms such as bacteria, yeast and fungi. These biological surface-active compounds exhibit potential antimicrobial activity. The present study aimed to isolate the biosurfactant producing yeast from fruits, vegetables and edible flowers (roses), and to evaluate the antibacterial activity. A total twenty yeast isolates were obtained and screened for biosurfactant production using oil displacement method and emulsification index (E₂₄). Among them, YNWaG exhibited the highest activity with oil displacement diameter of 5.16±0.05cm and emulsification index (E₂₄%) of 51.13±0.15. The biosurfactant produced by the selected yeast isolate YNWaG was further assessed for antibacterial activity against E. coli and S. aureus by agar well diffusion method. It was observed that the zone of inhibition increased with an increase in the concentration of biosurfactant. The zone of inhibition of E. coli increased from 27. 9±0.55 to 34.7±0.73 and for S. aureus from 16.1±0.45 to 31.5±0.50 with an increase in biosurfactant concentration from 1.25 mg/mL to 2.5 mg/mL. Statistical analysis using Student’s t-test indicates that the difference is highly significant (p < 0.001). Yeast derived biosurfactants are generally regarded as safe. The findings of this study shows that the yeast-derived biosurfactant can be used in healthcare, cosmetic industries and agriculture as nontoxic antimicrobial agents.

Keywords: Yeast-derived biosurfactant, Antibacterial activity, Agar well diffusion method, Zone of inhibition

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How to cite this article:

Nahida Fatima, Yahya Khan M. and Anuradha B. S. 2026. Isolation, Characterization and Antimicrobial activity of Biosurfactant Produced by Yeast isolate YNWaG. Int.J.Curr.Microbiol.App.Sci. 15(5): 70-76 doi: https://doi.org/10.20546/ijcmas.2026.1505.010

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Original Research Articles Volume : 15, Issue : 5, May, 2026

Isolation, Characterization and Antimicrobial activity of Biosurfactant Produced by Yeast isolate YNWaG

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