National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Herbal medicinal plants are widely used in traditional healthcare systems, but their safety and quality may be affected by microbial contamination during harvesting, processing, storage, and marketing. The present study was undertaken to investigate the fungal diversity associated with selected dried herbal medicinal plant samples. A total of ten herbal samples representing different plant parts, including rhizomes, leaves, stems, roots, and fruits, were collected from local herbal markets and analyzed for fungal contamination using the dilution plate technique. The fungal isolates were identified based on colony morphology and microscopic characteristics. The results revealed the presence of 17 fungal species belonging to several genera, including Aspergillus, Penicillium, Fusarium, Cladosporium, Rhizopus, and Mucor. Among the investigated samples, Terminalia belerica fruits exhibited the highest fungal load and diversity, with a total colony count of 313 and a mean value of 18.41 ± 10.72, whereas Acorus calamus rhizomes and Azadirachta indica leaves showed comparatively lower fungal contamination. The dominant fungal genera observed across the samples were Aspergillus, Penicillium, and Fusarium, which are commonly associated with stored plant materials and include several mycotoxin-producing species. The findings highlight the importance of proper post-harvest handling, hygienic processing, and suitable storage conditions to minimize fungal contamination and ensure the microbiological safety and quality of herbal medicines.
Agarwal A, Sharma S, Verma RK, Singh P (2021). Mycological assessment of fungal contamination in medicinal plants sold in herbal markets of North India. Journal of Applied Biology & Biotechnology, 9(4):120–126. https://doi.org/10.7324/JABB.2021.94016
A?tyn, I., & Twaru?ek, M. (2020). Mycotoxin contamination concerns of herbs and medicinal plants. Toxins, 12(3), 182. https://doi.org/10.3390/toxins12030182
Bennett, J. W., & Klich, M. (2003). Mycotoxins. Clinical Microbiology Reviews, 16(3), 497–516. https://doi.org/10.1128/CMR.16.3.497-516.2003
Bhattacharya K, Raha S (2020). Deteriorative changes of fungal contaminated medicinal herbs and their health implications. Indian Journal of Microbiology, 60(2):157–165. https://doi.org/10.1007/s12088-020-00852-5
Chaudhary S, Mehta P, Sharma R (2022). Microbial quality evaluation of herbal medicines marketed in India. Indian Journal of Pharmaceutical Sciences, 84(3):456–462. https://doi.org/10.36468/pharmaceutical-sciences.956
Chen L, Guo W, Zheng Y, Zhou J, Liu T, Chen W, Liang D, Zhao M, Zhu Y, Wu Q, Zhang J (2020). Occurrence and characterization of fungi and mycotoxins in contaminated medicinal herbs. Toxins, 12:30.
Ekor, M. (2014). The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4, 177. https://doi.org/10.3389/fphar.2013.00177
Haq IU, Taj R, Nafees M, Hussain A (2024). Mycotoxin detection in selected medicinal plants using chromatographic techniques. Biomedical Chromatography, 38:e5831.
Hu, M., Wang, L., Su, D., et al., (2024). Evaluation of mycotoxins, mycobiota and toxigenic fungi in traditional medicinal herbs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2024.1454683
Kalyoncu F (2012). Determination of fungal contamination in herbal medicinal plants sold in markets. African Journal of Microbiology Research, 6(22):466–471. https://doi.org/10.5897/AJMR11.1442
Kanabus, J., Bry?a, M., Le?nowolska-Wnuczek, K., et al., (2025). Mycotoxins occurrence in herbs, spices, dietary supplements and their exposure assessment. Toxins, 18(1), 20. https://doi.org/10.3390/toxins18010020
Kumar V, Roy BK, Kumar R (2023). Fungal diversity associated with stored medicinal plants and their potential mycotoxin risk. Journal of Applied and Natural Science, 15(2):617–624. https://doi.org/10.31018/jans.v15i2.4463
?tyn I, Twaru?ek M (2020). Mycotoxin contamination concerns of herbs and medicinal plants. Toxins, 12(3):182.
Pitt, J. I., & Hocking, A. D. (2009). Fungi and Food Spoilage (3rd ed.). Springer. https://doi.org/10.1007/978-0-387-92207-2
Raper KB, Fennell DI (1965). The Genus Aspergillus. Williams & Wilkins, Baltimore.
Samson, R. A., Houbraken, J., Thrane, U., Frisvad, J. C., & Andersen, B. (2010). Food and Indoor Fungi. CBS-KNAW Fungal Biodiversity Centre. https://doi.org/10.3114/sim.2010.64.01
Sharma, R., Gupta, S., & Sharma, A. (2021). Microbial contamination of medicinal plants: A review. Journal of Herbal Medicine, 25, 100405. https://doi.org/10.1016/j.hermed.2020.100405
Singh J, Singh P, Gupta R (2024). Evaluation of fungal contamination in traditional herbal medicines and their health implications. Journal of Applied Research on Medicinal and Aromatic Plants, 33:100448. https://doi.org/10.1016/j.jarmap.2023.100448
Singh, P., Kumar, V., & Sharma, M. (2024). Quality control of herbal medicines: Microbial contamination and safety concerns. Journal of Ethnopharmacology, 318, 116782. https://doi.org/10.1016/j.jep.2023.116782
Subapriya R, Nagini S (2005). Medicinal properties of neem leaves: A review. Current Medicinal Chemistry – Anti-Cancer Agents, 5:149–156.
Wei, G., Guo, X., Liang, Y., et al., (2023). Occurrence of fungi and mycotoxins in herbal medicines and rapid detection of toxin-producing fungi. Environmental Pollution, 333, 122082. https://doi.org/10.1016/j.envpol.2023.122082
Yang Z, Xue H, Han Y, Ding H, Zhang Y (2025). Research progress and management strategies for common mycotoxin contamination of traditional medicines. Journal of Fungi, 11:411![]() |
![]() |
![]() |
![]() |
![]() |