National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The laboratory studies were conducted at Department of Plant Pathology and Microbiology, Post Graduate Institute, MPKV, Rahuri for the isolation, purification and characterization of isolates obtained from rhizosphere of Sugarcane. Completely randomized design (CRD) was used for laboratory studies. The present investigation was carried out to isolate and characterize Gluconacetobacter and potassium mobilizing bacteria (KMB) from the rhizosphere and endosphere of sugarcane. A total of 11 Gluconacetobacter and 5 KMB isolates were obtained from different locations of Ahilyanagar district, Maharashtra. The isolates were characterized based on morphological, cultural, and biochemical characteristics. All isolates were Gram-negative, rod-shaped, motile, and showed positive KOH reaction. Cultural characterization revealed variation in colony morphology on different media, with isolates GSD?? and KSA?? showing better growth and mucoid nature. Functional evaluation indicated significant variation in nutrient mobilization, wherein Gluconacetobacter isolate GSD?? recorded the highest nitrogen fixation (25.40 µg ml?¹), while KMB isolate KSA?? showed maximum potassium solubilization (34 µg ml?¹) and solubilization index (3.77). The results indicate that these isolates have potential for use as bioinoculants in sustainable sugarcane cultivation.
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