National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Foodborne diseases (FBDs) represent a growing global health challenge, especially in the face of the growing circulation of bacteria that carry antimicrobial resistance mechanisms. Continuous monitoring of indicator microorganisms is a key factor in avoiding risks. Klebsiella pneumoniae is a Gram-negative bacterium of the Enterobacteriaceae family, considered opportunistic and responsible for serious infections in susceptible individuals. Its presence in food can represent a risk of FBDs. A sample of cooked chicken linked to an outbreak of gastroenteritis affecting 35 people in Itá Ibaté, Corrientes, Argentina, was analyzed. Coliform counts were performed using most probable number (MPN), isolation on Xylose Lysine Deoxycholate (XLD) agar, and molecular detection by endpoint PCR of K. pneumoniae, Salmonella spp. and Escherichia coli O157. The coliform count was > 2 400/g and amplicons of 908 bp specific to K. pneumoniae were obtained, confirming its presence as the only identified agent. Molecular characterization of the blaKPC2 gene was also performed by PCR and was not detected. A fragment of DNA from the isolate was sequenced to confirm the identity of the isolate found and compare it with previously reported reference sequences. The findings underscore the need to strengthen Good Manufacturing Practices (GMP) and epidemiological surveillance to prevent future outbreaks.
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