Optimization of Cultural Conditions for the Production of Cellulase from Agrowaste Using Penicillium brocae PSKV2 under Solid State Fermentation
1Department of Microbiology, School of Sciences, Maharani Science College for Women, Maharani Cluster University, Palace Road, Bengaluru- 560 001, Karnataka, India. 2aDepartment of Biotechnology, School of Applied Sciences, REVA University, Bengaluru - 560 064 Karnataka, India 2bCenter for Biotechnology and Molecular Biology, REVA Research Center, REVA University,Bengaluru - 560 064, Karnataka, India 2Department of Sericulture, School of Sciences, Maharani Science College for Women, Maharani Cluster University, Palace Road, Bengaluru- 560 001, Karnataka, India
*Corresponding author
Abstract:
Recent interest by researchers to produce cellulase from cheaper substrate is justifiable. Recently, agro residues have been used as carbon sources in cellulase production in industrial biotechnology. To investigate the production and optimization of cultural conditions for production of cellulase under solid state fermentation (SSF) from Penicillium brocae PSKV2 using surface-modified jack fruit waste and corncobs as substrate. The soil samples from the bottom of decaying agrowaste dumps from different locations of Bengaluru rural area for the isolation of fungal strain. The fungal isolates were screened for cellulase production. Major regional agro wastes such as Jack fruit waste, corn cobs, paddy straw, sugarcane bagasse were screened for production of cellulase. The cultural condition for optimum production of cellulase under SSF were optimized. Out of 60 fungal strains 6 fungal strains were positive for cellulolytic activity, only one positive fungal strain which showed clearer zone was selected for further work. The selected strain was named as Penicillium brocae PSKV2 after confirmation through automated genome sequencing. High enzyme activity was observed in jackfruit waste (23.96 U/mL) compared to corn cob (17.79 U/mL) and rice straw (11.40 U/mL). The optimum inoculum size for the production of cellulase enzyme was found to be 1mL showing enzyme activity of 27.38 U/mL at 5 days incubation period (15.06 U/mL), 65% moisture level (27.38 (U/mL), pH 6 (23.96 U/mL), and at 30°C temperature (27.38 U/mL). A novel fungal strain “Penicillium brocae PSKV2” was isolated from soil sample of decaying agrowaste dump in Bengaluru rural area. This pilot study finding could be explored in large scale industrial production of cellulase enzyme from cheaper agrowaste at the standardized optimum cultural conditions deduced in this study.
Keywords: Penicillium brocae PSKV2, SSF, Cellulase, Agrowaste, Jack fruit, Corncob
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How to cite this article:
Vishwanatha T., Keshavamurthy M., Manjula A. C. and Ahalya M. T. 2026. Optimization of Cultural Conditions for the Production of Cellulase from Agrowaste Using
Penicillium brocae PSKV2 under Solid State Fermentation.
Int.J.Curr.Microbiol.App.Sci. 15(3): 215-223. doi:
https://doi.org/10.20546/ijcmas.2026.1503.022
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National Academy of Agricultural Sciences (NAAS) 
