National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial vaginosis (BV), a common vaginal dysbiosis characterised by a disparity in the commensal of vaginal polymicrobial community. Facultative anaerobic bacteria are more often found in patients due to impaired immune system. However, the effective diagnosis and understanding the prevalence and type of bacteria in BV is essential for the treatment. To find out the frequency, distribution, and diversity of the bacterial isolates in vaginal samples of reproductive-age women who were diagnosed with BV. 150 vaginal swab samples were collected from reproductive-age women. Samples cultured in different agar media to isolate facultative anaerobic bacteria from the BV-positive samples. The isolated bacteria were identified by microscopic examination and biochemical tests, followed by a statistical analysis, viz., Shannon diversity index, Simpson’s diversity index, and species evenness, to determine the diversity and distribution of the bacterial species among the vaginal samples. Shannon and Simpson values indicate moderate species diversity, with dominance by bacteria such as Klebsiella spp., E. coli, and Enterococci spp. The use of culture and statistical analysis contributes for understanding the microbial community of the vagina in BV. They also provide insight into bacterial population stability, dominance patterns, and the extent of variation of microbial communities. This confirms bacterial vaginosis as a polymicrobial condition.
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