National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A crude oil tolerant bacterial strain, Bacillus spizizenii S1, was isolated from petroleum hydrocarbon contaminated soil and identified through 16S rRNA sequencing showing 98.3% similarity to Bacillus spizizenii and deposited in GenBank under the accession number PV533893. The bacterial strain produced a highly effective biosurfactant when grown on natural substrates, with cotton seed providing the highest yield of 54% at a 1.5% concentration. The biosurfactant showed multiple strong surface-active properties, including excellent oil displacement, foaming and emulsifying abilities. It also exhibited efficient hydrocarbon degradation in DCPIP assays. In soil column studies, treatment with 1% biosurfactant achieved 76 % crude oil removal within 10 days, performing significantly better than synthetic surfactants such as SDS and Triton X-100. GC-MS analysis revealed enhanced solubilization and breakdown of hydrophobic compounds in the treated soil. Phytotoxicity tests using black gram and maize showed better root and shoot growth, confirming its low toxicity and environmental safety. Overall, Bacillus spizizenii S1 and its biosurfactant demonstrate great potential as an eco-friendly and effective solution for cleaning up petroleum contaminated environments.
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