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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:7, July, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(7): 101-108
DOI: https://doi.org/10.20546/ijcmas.2025.1407.013


Isolation of Natural Microflora from Mulberry (Morus spp.) Fruits and Detailed Characterization of Lactic Acid Bacteria
S. Menaka1*, R. Kalpana2, M. Sabarishand M. Mithilasri4
1,2Forest College and Research Institute, TNAU, Tamil Nadu, India
3Department of Sericulture, Government of Tamil Nadu, Tamil Nadu, India
4Central Silk Board, Ministry of textiles, Government of India, Bangalore, India
*Corresponding author
Abstract:

This study investigated the natural microflora, including lactic acid bacteria (LAB), associated with fresh mulberry (Morus spp.) fruits collected from various regions of Tamil Nadu. Standard microbiological methods were used to enumerate bacteria, fungi, yeast, and LAB. Bacterial populations ranged from 6.09 to 7.29 log cfu g?¹, fungal counts from 2.65 to 2.87 log cfu g?¹, and yeast populations from 3.12 to 3.35 log cfu g?¹. LAB counts on MRS agar ranged between 4.72 to 6.87 log cfu g?¹. Four morphologically distinct LAB colonies were isolated, all Gram-positive and catalase-negative. Most isolates were rod-shaped, while 20% were cocci. The dominant colony type round, creamy, and raised with entire margins accounted for 54.42% of the isolates. These findings indicate that mulberry fruits harbour a diverse microflora, including LAB strains with potential applications in probiotic development and functional food fermentation.


Keywords: Mulberry fruit, isolation, microflora, lactic acid bacteria, purification, probiotics


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How to cite this article:

Menaka, S, R. Kalpana, M. Sabarish and Mithilasri, M. 2025. Isolation of Natural Microflora from Mulberry (Morus spp.) Fruits and Detailed Characterization of Lactic Acid Bacteria.Int.J.Curr.Microbiol.App.Sci. 14(7): 101-108. doi: https://doi.org/10.20546/ijcmas.2025.1407.013
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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