National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Plant phyllosphere, through the action of the enzyme pectin methyl esterase, which releases methanol as a byproduct of cell wall metabolism. Methylotrophs methane or methanol as their sole carbon source and reduce GHG emission from the atmosphere. In the present study, phyllosphere methylotrophic microbial community (PMMC) were isolated from the phyllosphere of rabi season crops. Out of the 16 PMMC isolates, six crops PMMC that is - Chickpea (POL 01), Chickpea (PUSA 3043), Chickpea (PUSA 3057), Radish (Pusa Himani), Beetroot (Crimson Globe) and Broad bean (Pusa Sumit) were selected based on their growth in Nitrate mineral salt medium (NMS) supplemented with 1% methanol. PMMC isolates were screened for plant growth promoting traits namely N2-fixation, P-solubilization, K-solubilization, and Zn-solubilization. PMMC of Beetroot and Raddish performed best compared to other PMMC in terms of their PGPR traits. The methanol use efficiency of six PMMC was also measured in terms of its conversion to CO2 which ranged from 3.74-7.48 mg of CO2/21 days. Based on the mesocosm experiment results, soil amended with the PMMC of Radish (Pusa Himani) significantly (P< 0.05) improved the available N, P, K and Soil organic carbon percentage at 21 days of incubation as compared to non-inoculated PMMC soils. The positive relationship between soil enzyme activities like phenol oxidase (POX), FDA hydrolase, β-glucosidase and dehydrogenase activities involved in C cycling in soil underlines the role of PMMC of different crops in plant growth promotion and sequestration of methane in the soil as microbial necromass.
Ahmad, F., Ahmad, I., & Khan, M. S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological research, 163(2), 173-181. https://doi.org/10.1016/j.micres.2006.04.001.
Aimen, H., Khan, A. S., & Kanwal, N. (2018). Methanotrophs: The natural way to tackle greenhouse effect. Jounal of Bioremediation & Biodegradation, 9(2), 432. https://doi.org/10.4172/2155-6199.1000432.
Bai, B., Yang, X., Zhao, Q., Liu, R., & Ren, J. (2020). Inoculations with Pseudomonas fluorescens and Bacillus cereus affect the soil enzyme activity, growth and rhizosphere microbial diversity of Taxus chinensis var. mairei. Plant and Soil, 455, 41-52. https://doi.org/10.1007/s11104-020-04660-8.
Bray, R. H. and Kurtz, L. T. (1945). Determination of Total Organic and Available Forms of Phosphorus in Soils. Soil Science, 59, 39-45. http://dx.doi.org/10.1097/00010694-194501000-00006
Casida Jr, L. E. (1977). Microbial metabolic activity in soil as measured by dehydrogenase determinations. Applied and environmental microbiology, 34(6), 630-636. https://doi.org/10.1128/aem.34.6.630-636.1977.
Delmotte, N., Knief, C., Chaffron, S., Innerebner, G., Roschitzki, B., Schlapbach, R.,... & Vorholt, J. A. (2009). Community proteogenomics reveals insights into the physiology of phyllosphere bacteria. Proceedings of the National Academy of Sciences, 106(38), 16428-16433. https://doi.org/10.1073/pnas.0905240106
Eivazi, F., & Zakaria, A. (1993). β-Glucosidase activity in soils amended with sewage sludge. Agriculture, ecosystems & environment, 43(2), 155-161. https://doi.org/10.1016/0167-8809(93)90117-8.
Galbally, I. E., & Kirstine, W. (2002). The production of methanol by flowering plants and the global cycle of methanol. Journal of Atmospheric Chemistry, 43, 195-229. https://doi.org/10.1023/A:1020684815474.
Gayan, A., Phukan, S., Bhattacharyya, A., Sonowal, D., Dutta, N., & Nath, D. J. (2023). Leaf Surface Colonizing Pink Pigmented Facultative Methylotrophs Harnessed for Their Plant Growth Promoting Traits. Journal of the Indian Society of Soil Science, 71(3), 342-350. http://dx.doi.org/10.5958/0974-0228.2023.00031.2.
Gayathry, G., Sabarinathan, K. G., & Jayalakshmi, T. (2024). Phyllosphere Microbiome in Ecosystem Management and Plant Growth Promotion for Agricultural Sustainability. International Journal of Ecology and Environmental Sciences. https://doi.org/10.55863/ijees.2024.0300.
Green, V. S., Stott, D. E., & Diack, M. (2006). Assay for fluorescein diacetate hydrolytic activity: optimization for soil samples. Soil Biology and Biochemistry, 38(4), 693-701. https://doi.org/10.1016/j.soilbio.2005.06.020.
Hanway, J. J. (1952). Soil analysis methods as used in the iowa state college soil testing laboratory. Iowa agriculture, 57, 1.
Hendel, B., Sinsabaugh, R. L., & Marxsen, J. (2020). Lignin-degrading enzymes: phenoloxidase and peroxidase. Methods to study litter decomposition: a practical guide, 425-431. https://doi.org/10.1007/978-3-030-30515-4_46.
Jamir, E., Kangabam, R. D., Borah, K., Tamuly, A., Deka Boruah, H. P., Silla, Y., 2019. Role of soil microbiome and enzyme activities in plant growth nutrition and ecological restoration of soil health.in: Kumar, A., Sharma, S. (Eds.), Microbes and enzymes in soil health and bioremediation (pp. 99-132). Singapore: Springer. https://doi.org/10.1007/978-981-13-9117-0_5pp99-132.
Joel, G. V. V., Latha, P. C., Gopal, A. V., & Sreedevi, B. (2023). Isolation and characterization of pink pigmented facultative methylotrophic bacteria: An in-vitro evaluation of the isolates for plant growth promotion on rice. Biological Forum–An International Journal. 15(2), 1167-1179.
Ju, W., Liu, L., Fang, L., Cui, Y., Duan, C., & Wu, H. (2019). Impact of co-inoculation with plant-growth-promoting rhizobacteria and rhizobium on the biochemical responses of alfalfa-soil system in copper contaminated soil. Ecotoxicology and Environmental Safety, 167, 218-226. https://doi.org/10.1016/j.ecoenv.2018.10.016.
Kanukollu, S., Remus, R., Rücker, A. M., Buchen-Tschiskale, C., Hoffmann, M., & Kolb, S. (2022). Methanol utilizers of the rhizosphere and phyllosphere of a common grass and forb host species. Environmental Microbiome, 17(1), 35. https://doi.org/10.1186/s40793-022-00428-y.
Khati, P., Chaudhary, P., Gangola, S., Bhatt, P., & Sharma, A. (2017). Nanochitosan supports growth of Zea mays and also maintains soil health following growth. 3 Biotech, 7, 1-9. https://doi.org/10.1007/s13205-017-0668-y
Kögel-Knabner, I. (2002). The macromolecular organic composition of plant and microbial residues as inputs to soil organic matter. Soil biology and biochemistry, 34(2), 139-162. https://doi:10.1016/j.soilbio.2016.08.011.
Li, H., Qiu, Y., Yao, T., Ma, Y., Zhang, H., & Yang, X. (2020). Effects of PGPR microbial inoculants on the growth and soil properties of Avena sativa, Medicago sativa, and Cucumis sativus seedlings. Soil and Tillage Research, 199, 104577. https://doi.org/10.1016/j.still.2020.104577.
Liang, C., Amelung, W., Lehmann, J., & Kästner, M. (2019). Quantitative assessment of microbial necromass contribution to soil organic matter. Global change biology, 25(11), 3578-3590. https://doi.org/10.1111/gcb.14781.
Liu, J., Wang, J., Morreale, S. J., Schneider, R. L., Li, Z., & Wu, G. L. (2023). Contributions of plant litter to soil microbial activity improvement and soil nutrient enhancement along with herb and shrub colonization expansions in an arid sandy land. Catena, 227, 107098. https://doi.org/10.1016/j.catena.2023.107098.
Madhaiyan, M., Alex, T. H. H., Ngoh, S. T., Prithiviraj, B., & Ji, L. (2015). Leaf-residing Methylobacterium species fix nitrogen and promote biomass and seed production in Jatropha curcas. Biotechnology for biofuels, 8, 1-14. https://doi.org/10.1186/s13068-015-0404-y.
Meena, K. K., Kumar, M., Kalyuzhnaya, M. G., Yandigeri, M. S., Singh, D. P., Saxena, A. K., & Arora, D. K. (2012). Epiphytic pink-pigmented methylotrophic bacteria enhance germination and seedling growth of wheat (Triticum aestivum) by producing phytohormone. Antonie Van Leeuwenhoek, 101, 777-786. https://doi.org/10.1007/s10482-011-9692-9.
Murrell, J. C., McDonald, I. R., & Bourne, D. G. (1998). Molecular methods for the study of methanotroph ecology. FEMS Microbiology Ecology, 27(2), 103-114. https://doi.org/10.1111/j.1574-6941.1998.tb00528.x.
Nitika, T. K., & Mehta, D. K. (2018). Soil nutrient status, NPK uptake and viable bacterial count as influenced by different organic nutrient sources in European carrot. International Journal of Current Microbiology and Applied Sciences, 7(8), 1783-1789. https://doi.org/10.20546/ijcmas.2018.708.204
Rani, V., Bhatia, A., Nain, L., Tomar, G. S., & Kaushik, R. (2021). Methane utilizing plant growth-promoting microbial diversity analysis of flooded paddy ecosystem of India. World Journal of Microbiology and Biotechnology, 37, 1-22. https://doi.org/10.1007/S11274-021-03018-1.
Saha, S., Prakash, V., Kundu, S., Kumar, N., & Mina, B. L. (2008). Soil enzymatic activity as affected by long term application of farm yard manure and mineral fertilizer under a rainfed soybean–wheat system in NW Himalaya. European Journal of Soil Biology, 44(3), 309-315. https://doi.org/10.1016/j.ejsobi.2008.02.004.
Sahoo, K. K., Goswami, G., & Das, D. (2021). Biotransformation of methane and carbon dioxide into high-value products by methanotrophs: current state of art and future prospects. Frontiers in microbiology, 12, 636486. https://doi.org/10.3389/fmicb.2021.636486.
Smita, M., & Goyal, D. (2017). Isolation and characterization of free-living nitrogen fixing bacteria from alkaline soils. International Journal of Scientific World, 5(1), 18-22. https://doi.org/10.14419/ijsw.v5i1.6936.
Solanki, J. P., Vyas, R. V., Jhala, Y. K., & Patel, H. K. (2024). Development of Pink Pigmented Facultative Methylotrophs’(PPFMs) Consortium Formulation and Its Efficacy on Chilli (Capsicum annuum). Journal of Advances in Microbiology, 24(3), 1-7. https://doi.org/10.9734/jamb/2024/v24i3801.
Stotzky G., 2016. Microbial respiration, in: Norma A.G. (Eds.), Methods of Soil Analysis, Part 2: Chemical and Microbiological Properties. American Society of Agronomy, Inc. Madison, 9, 1550–1572. https://doi.org/10.2134/AGRONMONOGR9.2.C62.
Subbiah, B. V., & Asija, G. L. (1956). A rapid procedure for the estimation of available nitrogen in soils, Current Science, 25, 259-260.
Sultana, N., Jun, Z. H. A. O., Yuanfeng, C. A. I., Rahman, G. M., Alam, M. S., Faheem, M.,... & Zhongjun, J. I. A. (2022). Methanotrophy-driven accumulation of organic carbon in four paddy soils of Bangladesh. Pedosphere, 32(2), 348-358. https://doi.org/10.1016/S1002-0160(20)60030-3.
Vadivukkarasi, P., & Bhai, R. S. (2020). Phyllosphere-associated Methylobacterium: a potential biostimulant for ginger (Zingiber officinale Rosc.) cultivation. Archives of microbiology, 202(2), 369-375. https://doi.org/10.1007/s00203-019-01753-6.
Walkley, A., & Black, I. A. (1934). An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil science, 37(1), 29-38.
Wang, X., Song, D., Liang, G., Zhang, Q., Ai, C., & Zhou, W. (2015). Maize biochar addition rate influences soil enzyme activity and microbial community composition in a fluvo-aquic soil. Applied soil ecology, 96, 265-272. https://doi.org/10.1016/j.apsoil.2015.08.018.
Yurimoto, H., & Sakai, Y. (2023). Interaction between C1-microorganisms and plants: contribution to the global carbon cycle and microbial survival strategies in the phyllosphere. Bioscience, Biotechnology, and Biochemistry, 87(1), 1-6. https://doi.org/10.1093/bbb/zbac176.
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