National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacillus thuringiensis (Bt) is a main producer of insecticidal proteins but it has also a good potential to produce antibacterial proteins like bacteriocins. In this study, the bacteriocin production potential of native Bt strains in our collection were investigated. The antimicrobial activity of 100 Bt strains were screened by agar spot assay and 17 of them with clear inhibition zones were further analysed by well diffusion assay. Among them, Bt-28Q1 strain resulted in a highest inhibition zone against Bacillus cereus. The antimicrobial substance was detected at the highest level at the beginning of logarithmic phase in the growth curve. Its production was at the maximum level in Tryptic soy broth (TSB) compared to other medium. The antimicrobial substance was heat-stable, pH-stable (range 5-9), and proteinase K sensitive, similar to bacteriocins. This bacteriocin was partially purified with ammonium sulfate precipitation and its molecular weight was determined as around 12 kDa via SDS-PAGE and gel overlay assays. Bacteriocin showed antimicrobial activity to most of the Bacillus species and anticancer activity against human cancer cell lines A549 and HT-29. Sequence analysis of 16S rDNA and flagellin hag genes of the Bt 28Q1 strain identified it as Bacillus thuringiensis subsp. tochigiensis. These findings indicate that bacteriocin from Bt-28Q1 has potency for being used as bioactive molecule to prevent the growth of food spoilage bacteria and for development of a new anticancer agent.
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