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Original Research Articles                      Volume : 13, Issue:8, August, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(8): 132-145
DOI: https://doi.org/10.20546/ijcmas.2024.1308.017


Evaluation of chemically characterized Ocimum tenuiflorum L. essential oil as phytopreservative against fungal deterioration of Justicia adhatoda L. raw materials during storage
Rajneesh Rao1, Sanyogita Kumari2 and Ashok Kumar1*
1Laboratory of Mycotoxin & Botanical Pesticides, Department of Botany,
Deen Dayal Upadhyay Gorakhpur University, Gorakhpur-273009, India
2Raghunath Girl’s Post Graduate College, Meerut-250001, India
*Corresponding author
Abstract:

The stored herbal raw materials (HRMs) of Justicia adhatoda were found associated with spores of various storage fungi in which Cladosporium cladosporioides exhibited the highest relative density (25.02%) followed by Aspergillus flavus (22.49%) and A. niger (21.46%). The chemical composition of Ocimum tenuiflorum essential oil (OtEO) showed 41 considerable peaks. Eugenol (61.30%) was found as major component followed by β- Caryophyllene (11.89%), Germacrene D (9.14%), α- Cubebene (2.54%) and Carvacrol (2.04%). OtEO showed broad spectrum fungitoxicity and MIC against A. flavus DDUGU-07 was recorded at 0.5 mg/ml while aflatoxin B1 production was completely checked at 0.3 mg/ml. The high performance thin layer chromatography (HPTLC) analysis of fumigated HRMs of J. adhatoda reveals that OtEO was found effective in control the degradation or chemical transformation of medicinal component, vasicine found in J. adhatoda. Hence, OtEO having exclusive merit to possessing antifungal, antiaflatoxigenic and broad fungitoxic spectrum strengthening its safe exploitation as green preservative.


Keywords: Ocimum tenuiflorum, Essential oil, Aspergillus flavus, Aflatoxin B1, Antifungal, HPTLC


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How to cite this article:

Rajneesh Rao, Sanyogita Kumari and Ashok Kumar. 2024. Evaluation of chemically characterized Ocimum tenuiflorum L. essential oil as phytopreservative against fungal deterioration of Justicia adhatoda L. raw materials during storage.Int.J.Curr.Microbiol.App.Sci. 13(8): 132-145. doi: https://doi.org/10.20546/ijcmas.2024.1308.017
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