National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study assessed microbial populations in soil samples from the Bhopal, Capital, Madhya Pradesh, India using traditional dilution plating, supplemented with qPCR for molecular insights. The CFU counts ranged from 6.3 to 8.2 log10 CFU/gm, with sample 7 having the highest and sample 4 the lowest bacterial load, highlighting variability due to soil properties and environmental conditions. qPCR analysis showed an inverse relationship between CFU counts and CT values, confirming higher DNA concentrations with higher viable bacterial counts. This dual approach of combining CFU and qPCR methods offers a comprehensive understanding of microbial presence, consistent with prior research findings. High bacterial counts, as seen in sample 7, suggest healthy soil with robust nutrient cycling and organic matter decomposition, while lower counts in sample 4 may indicate less favorable conditions for microbial growth. Future studies should incorporate advanced molecular techniques, like high-throughput sequencing, to fully capture soil microbial diversity.
Aislabie, J., Deslippe, J. R., & Dymond, J. (2013). Soil microbes and their contribution to soil services. Ecosystem services in New Zealand–conditions and trends. Manaaki Whenua Press, Lincoln, New Zealand, 1(12), 143-161.
Amann, R. I., Ludwig, W., & Schleifer, K. H. (1995). "Phylogenetic identification and in situ detection of individual microbial cells without cultivation". Microbiological Reviews, 59(1), 143-169. https://doi.org/10.1128/mr.59.1.143-169.1995
Atlas Ronald, M. 1984. Microbiology: fundamental and application. Maxwell Macmillan Publishing, Canada. Pp. 987.
Coleman, D. C., Callaham, M. A., & Crossley Jr, D. A. (2017). Fundamentals of soil ecology. Academic press.
Daniel, R. (2005). "The metagenomics of soil". Nature Reviews Microbiology, 3(6), 470-478. https://doi.org/10.1038/nrmicro1160
Dominati E, Patterson M., MacKay A. 2010. A framework for classifying and quantifying natural capital and ecosystem services of soils. Ecological Economics 69: 1858–1868. https://doi.org/10.1016/j.ecolecon.2010.05.002
Fierer, N., & Jackson, R. B. (2006). The diversity and biogeography of soil bacterial communities. Proceedings of the National Academy of Sciences, 103(3), 626-631. https://doi.org/10.1073/pnas.0507535103
Ritz K, McHugh M, Harris J., 2003. Biological diversity and function in soils: contemporary perspectives and implications in relation to the formulation of effective indicators. OECD Expert Meeting on Soil Erosion and Soil Biodiversity Indicators, Rome, March 2003. Pp. 1–11.
Robertson,S., and Egger, K. 2010. BIOL203, Microbiology Laboratory Manual, UNBC
Torsvik, V., & Øvreås, L. (2002). "Microbial diversity and function in soil: from genes to ecosystems". Current Opinion in Microbiology, 5(3), 240-245. https://doi.org/10.1016/s1369-5274(02)00324-7
Torsvik, V., Goksoyr, J., & Daae, F. L. (1990). "High diversity in DNA of soil bacteria". Applied and Environmental Microbiology, 56(3), 782-787. https://doi.org/10.1128/aem.56.3.782-787.1990
Van Elsas, J. D., Trevors, J. T., & Wellington, E. M. (2006). Modern Soil Microbiology. CRC Press. https://doi.org/10.1201/9781420015201
Whitman, W. B., D. C. Coleman, W. J. Wiebe. Prokaryotes: the unseen majority Proc. Natl. Acad. Sci., 95 (1998), pp. 6578-6583 https://doi.org/10.1073/pnas.95.12.6578
Woese C, Kandler O, Wheelis M 1990. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proceedings of the National Academy of Sciences (USA) 87: 4576–4579. https://doi.org/10.1073/pnas.87.12.4576
Citations |
 |
 |
 |
 |
