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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 13, Issue:5, May, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(5): 183-193
DOI: https://doi.org/10.20546/ijcmas.2024.1305.024


Haematological Indices of Plasomodium berghei NK65 Infected Mice Treated with Ethanol Extract of Arthropteris palisotii
Magdalene Obi Abang1, Jonathan Osine Enyike1*, Victor Ejim1 and Josephine Eneji Egbung2
1Department of Biochemistry, University of Cross River State, P.M.B 1123, Calabar, Nigeria 2Department of Biochemistry, University of Calabar, P.M.B 1115, Calabar, Nigeria
*Corresponding author
Abstract:

Malaria continues to be a major global health issue, with more than three hundred million infections and one million deaths every year. Despite extensive efforts to control the disease, drug resistance and the emergence of new Plasmodium strains remain persistent challenges, necessitating the exploration of innovative therapeutic strategies. The study investigated the impact of varying doses of Arthropteris palisotii extract on some haematological indices in Swiss albino mice infected with Plasmodium berghei NK65. Swiss albino mice were inoculated intra-peritoneally with Plasmodium berghei NK65. The mice were grouped into six groups, six mice per group. Group NC were not infected with P.berghei, Group Neg.C and SD served as both negative and standard controls respectively, while Group LD, MD, and HD were treated with 100, 200, and 400 mg/kg body weight of Arthropteris palisotii extract respectively. After treatment period, all mice were sedated with ketamine and dissected. Blood obtained by cardiac puncture using EDTA treated bottles, sterile syringes and needles. Hematological indices were measured using standard methods. The extract caused 14.3%, 36.3% and 69.2% suppression in parasitaemia at 100mg/kg, 200mg/kg and 400mg/kg body weight respectively while Artesunate exerted 10.1% suppression at 5mg/kg body weight. There were significant decreases (P<0.05) in parasite density in the groups treated with extract of Arthropteris palisotii compared to the untreated group (Neg.C). Arthropteris palisotii exhibited significant performance in enhancing mild erythropoiesis, as well as in protecting blood cells from potential infections resulting from the inflammatory responses by Plasmodium bergheiin malaria conditions.


Keywords: Malaria, Arthropteris palisotii, Haematological indices


References:

Asangha, E. E., G. O. Igile, I. A. Iwara, P. E. Ebong and Eseyin, O. A. (2017). Hematological Indices of Plasmodium berghei Infected Mice Treated with Ethanol Extract and Fractions of Nauclea latifolia Roots. Int.J.Curr.Microbiol.App.Sci. 6(12): 2546-2556. https://doi.org/10.20546/ijcmas.2017.612.295

Bessman J. D. (1983). Improved classification of anemias by MCV and RDW.Amer J ClinPathol; 80: 322 - 6. https://doi.org/10.1093/ajcp/80.3.322 Clark, A. M. (2001). Natural Products as a Source for New Drugs. Pharmaceutical Research. 13:1133- 1144. https://doi.org/10.1023/a:1016091631721

Cyril-Olutayo, M C (2013). Effects of Angeissus leiocarpus on hematological parameters of mice infected with Plasmodium berghei. J Plant Studies, 2(2): 13 – 21. https://doi.org/10.5539/jps.v2n2p13

Erhart, L. M., Yingyuen, K., Chuanak, N., Buathong, N., Laoboonchai, A., Miller, R. S., Meshnick, S. R., Gasser, R. A. and Wongsrichanalai, C. (2004). Hematological and clinical indices of malaria in a semi-immune population of Western Thailand. American Journal of Tropical Medicine and Hygiene, 70, 8 – 14.

Gravigan, C. S., Dalton, S. P. and Bell, A. (2001). The role of aminopeptidases in hemoglobin degradation in Plasmodium falciparum-infected erythrocytes. Molecular Biochemistry and Parasitology, 117(1), 37 – 48. https://doi.org/10.1016/s0166-6851(01)00327-9

Hassler, M. (2020). World Ferns: Checklist of Ferns and Lycophytes of the World (version Nov 2018). In: Species 2000 & ITIS Catalog of Life, 2019 Annual Checklist (Roskov Y., Ower G., Orrell T., Nicolson D., Bailly N., Kirk P M, Bourgoin T., DeWalt R E, Decock W., Nieukerken E van, Zarucchi J., Penev L., eds.). Species 2000: Naturalis, Leiden, the Netherlands. 2405-884. https://doi.org/10.15468/qrjvbc

Klayman, D. L. (1985). Qinghaosu (artemisinin): an antimalarial drug from China. Science, 228, 1049 – 1055. https://doi.org/10.1126/science.3887571

Les, R. (2011). Field Guide to the Native Plants of Sydney. 978-0-7318-1211-0. 309

Momoh, J., Longe, A. O. and Campbell, C. A. (2014). In vivo antiplasmodial and In vitro antioxidant activity of ethanolic leaf extract of Alstonia boonie (EWE AHUN) and its effects on some biochemical parameters in Swiss albino mice infected with Plasmodium berghei NK 65. European Scientific Journal, 10 (18), 68 – 82.

NIH, 1985

Obi-Abang M., Enyike J. O Margaret A. E., Godwin E. E. (2020). Effect of Ficus glumosa Delile Leaves on some Biochemical and Haematological Indices of Rats Fed with Kerosene Contaminated Feed. Tropical Journal of Natural Product Research 4(12):1154-1160

Odaibo, A. B. & Igbeneghu, C. (2013). Impact of acute malaria on some haematological parammeters in a semi-urban community in South-Western Nigeria. ActaParasitologicaGlobalis, 4 (1), 1- 5. https://doi.org/10.5829/idosi.apg.2013.4.1.71233

Okoroiwu, I. L., Obeagu, E. I., Elemchukwu, Q. and Ochei, K. C. (2014). Some hematological parameters in malaria parasitaemia. IOSR Journal of Dental and Medical Sciences, 13 (9), 74 – 77. https://doi.org/10.9790/0853-13937477

Ovuakporaye, S. I. (2011). Effect of malaria parasite on some haematological parameters: red blood cell count, packed cell volume and haemoglobin concentration. Journal of Medical and Applied Biosciences, 3, 1 - 7.

Schnell, M. A., Hardy, C. and Wilson, J. (2002). Effect of blood collection technique in mice on clinical pathology parameters. Human Gene Therapy, 13 (1), 155-61. https://doi.org/10.1089/10430340152712700.

Snow, R. W., Guera, C. A., Noov, A. M., Myint, H. Y and Hays, A. (2005): The global distribution of clinical episodes of Plasmodium falciparium malaria. Mar 10;434(7030):214-7. https://doi.org/10.1038/nature03342

Taha, K., El-Dein, S. D., Idrees, M., Makboul, G. and Baidas, G. (2007). Hematological changes in malaria in relation to Plasmodium species. Kuwait Medical Journal, 39 (3), 262 – 267.

World Health Organization. (2018). World Malaria Report 2018. WHO; Geneva, Switzerland


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How to cite this article:

Magdalene Obi-Abang, Jonathan Osine Enyike, Victor Ejim and Josephine Eneji Egbung. 2024. Haematological Indices of Plasomodium berghei NK65 Infected Mice Treated with Ethanol Extract of Arthropteris palisotii.Int.J.Curr.Microbiol.App.Sci. 13(5): 183-193. doi: https://doi.org/10.20546/ijcmas.2024.1305.024
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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