National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Earthworm coelomic fluid has garnered attention for its potential anticancer properties, with studies primarily concentrated in China and India. This research investigates the extraction methods, protein concentration, and effects of coelomic fluid from Eisenia fetida on breast cancer cell lines MCF-7 and 4T1. The warm water shock and electric shock methods were compared, revealing differences in yield and protein concentration. While the electric shock method was more efficient in extraction, the warm water shock method yielded higher protein concentration. Both methods inhibited cell growth, with the warm water shock method exhibiting slightly superior results. Regression analysis showed concentration- and time-dependent responses, with optimal effectiveness observed at lower concentrations and within the initial 24 hours. Comparative analysis indicated comparable inhibitory effects between extraction methods. Our findings contribute to understanding the anticancer properties of earthworm coelomic fluid and its potential as a therapeutic agent. Previous studies have demonstrated its apoptotic induction of cancer cells and synergistic effects with chemotherapeutic agents. Further research is needed to explore its efficacy in clinical settings and potential in combination therapies.
“American Cancer Society,” Cancer facts & figures., 2008.
“Breast Cancer: What You Need To Know Before Treatment,” NationalCancer Institute., pp. 00-1556, 2003.
Allred, D. C. “Ductal carcinoma in situ: terminology, classification, and natural history,” J. Natl. Cancer Inst. Monogr., vol. 2010, no. 41, pp. 134–138, 2010.
Ambudkar, S. V., C. Kimchi-Sarfaty, Z. E. Sauna, and M. M. Gottesman, “P-glycoprotein: from genomics to mechanism,” Oncogene, vol. 22, no. 47, pp. 7468–7485, 2003. https://doi.org/10.1038/sj.onc.1206948
Augustine, D., R. Rao, A. Jayaraman, and K. N. Chidambara Murthy, “Anti-proliferative activity of earthworm coelomic fluid using oral squamous carcinoma KB 3-1 cells: An In vitro study with serine protease analysis,” Pharmacogn. Mag., vol. 14, no. 59, p. 528, 2018. https://doi.org/10.4103/pm.pm_412_18
Augustine, D., R. Rao, J. Anbu, and K. N. Chidambara Murthy, “Anticancer prospects of earthworm extracts: A systematic review of In vitro and In vivo studies,” Pharmacogn. Rev., vol. 12, no. 23, p. 46, 2018.
Augustine, D., R. Rao, J. Anbu, and K. N. Chidambara Murthy, “In vitro antiproliferative effect of earthworm coelomic fluid of Eudrilus eugeniae, Eisenia foetida, and Perionyx excavatus on squamous cell carcinoma-9 cell line: A pilot study,” Pharmacognosy Res., vol. 9, no. 5, p. 61, 2017. https://doi.org/10.4103/pr.pr_52_17
Augustine, D., R. S. Rao, J. Anbu, and K. N. Chidambara Murthy, “In vitro cytotoxic and apoptotic induction effect of earthworm coelomic fluid of Eudrilus eugeniae, Eisenia foetida, and Perionyx excavatus on human oral squamous cell carcinoma-9 cell line,” Toxicol. Rep., vol. 6, pp. 347–357, 2019. https://doi.org/10.1016/j.toxrep.2019.04.005
Bernardini, S., A. Tiezzi, V. LaghezzaMasci, and E. Ovidi, “Natural products for human health: an historical overview of the drug discovery approaches,” Nat. Prod. Res., vol. 32, no. 16, pp. 1926–1950, 2018.
Bradford, M. M, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical biochemistry., vol. 72, no. (1-2), pp. 248-254, 1976. https://doi.org/10.1006/abio.1976.9999
Danaei, G., S. Vander Hoorn, A. D. Lopez, C. J. L. Murray, M. Ezzati, and Comparative Risk Assessment collaborating group (Cancers), “Causes of cancer in the world: comparative risk assessment of nine behavioural and environmental risk factors,” Lancet, vol. 366, no. 9499, pp. 1784–1793, 2005. https://doi.org/10.1016/S0140-6736(05)67725-2
de Martel C. et al., “Global burden of cancers attributable to infections in 2008: a review and synthetic analysis,” Lancet Oncol., vol. 13, no. 6, pp. 607–615, 2012. https://doi.org/10.1016/S1470-2045(12)70137-7
Dinesh, M. S., S. Sridhar, P. G. Chandana, V. Pai, K. S. Geetha, and R. N. Hegdge, “Anticancer Potentials of Peptides of Coelomic Fluid of Earthworm Eudrilus eugeniae,” Biosci. Biotechnol. Res. Asia, vol. 10, no. 2, pp. 601–606, 2013.
Dittmer, J., I. Oerlecke, and B. Leyh, “Involvement of mesenchymal stem cells in breast cancer progression,” in Breast Cancer - Focusing Tumor Microenvironment, Stem cells and Metastasis, InTech, 2011. https://doi.org/10.5772/21325
DuPré, S. A., D. Redelman, and K. W. Hunter Jr, “The mouse mammary carcinoma 4T1: characterization of the cellular landscape of primary tumours and metastatic tumour foci: Cellular landscape of mammary carcinoma 4T1,” Int. J. Exp. Pathol., vol. 88, no. 5, pp. 351–360, 2007. https://doi.org/10.1111/j.1365-2613.2007.00539.x
Edwards, C. A. and P. J. Bohlen, “Biology and ecology of earthworms,” Biology and ecology of earthworms, vol. 3, 1996.
Fiaschi, T. and P. Chiarugi, “Oxidative stress, tumor microenvironment, and metabolic reprogramming: a diabolic liaison,” Int. J. Cell Biol., vol. 2012, p. 762825, 2012. https://doi.org/10.1155/2012/762825
Fio?ka, Marta J., Jolanta Rzymowska, SylwiaBilska, KingaLewtak, Magdalena Dmoszy?ska?Graniczka, Krzysztof Grzywnowicz, Wojciech Ka?mierski, and Teresa Urbanik?Sypniewska. "Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells," Apmis 127.,vol. 127, no. 9, pp. 435-448, 2019. https://doi.org/10.1111/apm.12941
Gottesman, M. M. “How cancer cells evade chemotherapy: sixteenth Richard and Hinda Rosenthal Foundation Award Lecture,” Cancer Res., vol. 53, no. 4, pp. 747–754, 1993.
Horwitz, K. B., M. E. Costlow, and W. L. McGuire, “MCF-7: A human breast cancer cell line with estrogen, androgen, progesterone, and glucocorticoid receptors,” Steroids, vol. 26, no. 6, pp. 785–795, 1975. https://doi.org/10.1016/0039-128x(75)90110-5
Irma, J. H. and J. H. Beijnen, “Combined action and regulation of phase II enzymes and multidrug resistance proteins in multidrug resistance in cancer,” Cancer treatment reviews, vol. 34, pp. 505–520, 2008.
Macdonald, F., C. Ford, and A. Casson, Molecular biology of cancer, 2nd ed. London, England: Taylor & Francis, 2004. https://doi.org/10.4324/9780203503447
McGuire, S., “World cancer report 2014. Geneva, Switzerland: World health organization, international agency for research on cancer, WHO press, 2015,” Adv. Nutr., vol. 7, no. 2, pp. 418–419, 2016. https://doi.org/10.3945/an.116.012211
Nelson, N. J. “Migrant studies aid the search for factors linked to breast cancer risk,” J. Natl. Cancer Inst., vol. 98, no. 7, pp. 436–438, 2006.
Nicholas Zdenkowski, P. Butow, S. Tesson, and F. Boyle, “A systematic review of decision aids for patients making a decision about treatment for early breast cancer,” Breast, vol. 26, pp. 31–45, 2016. https://doi.org/10.1016/j.breast.2015.12.007
Patil, S. R. and P. M. Biradar, “Earthworm’s coelomic fluid: extraction and importance,” International Journal of Advanced Scientific Research, vol. 2, no. 2, pp. 1–04, 2017.
Permana et al., (2018) “Cytotoxic effects and anti-proliferative cancer activity of coelomic fluid from Lumbricus rubellus promotes apoptosis and reduces G2/M phase progression in HT-29 cells,” J. Appl. Pharm. Sci., vol. 8, no. 11, pp. 28–34, 2018. https://doi.org/10.7324/JAPS.2018.81104
Permana, Sofy, Reyudzky Putri Fityanti, Eviana Norahmawati, Agustin Iskandar, Erika Desy Anggraini Mulyadi, and Agustina Tri Endharti. "Coelomic Fluid of Eisenia fetida Ameliorates Cetuximab to Reduce K-Ras and Vimentin Expression through Promoting RUNX3 in an AOM/DSS-Induced Colitis Associated Colon Cancer." Evidence-Based Complementary and Alternative Medicine 2020.,2020. https://doi.org/10.1155/2020/9418520
Porter, P. L. “Global trends in breast cancer incidence and mortality,” SaludPublica Mex., vol. 51, pp. s141–s146, 2009.
Rathi, S. G. “In-vitro cytotoxic screening of Glycyrrhiza glabra L. (Fabaceae): A natural anticancer drug,” Journal of Young Pharmacists, vol. 1, no. 3, 2009. https://doi.org/10.4103/0975-1483.57071
Sadek, Shimaa A., Moshera G. Sayed, Sohair R. Fahmy, and Amel M. Soliman. "A Coelomic Fluid of Allolobophora caliginosa as Novel Prospects for Medicinal Antioxidants, Anti-inflammatory, Antiproliferative, Analgesics, and Antipyretics." 2022.
Schweizer, R., W. Tsuji, V. S. Gorantla, K. G. Marra, J. P. Rubin, and J. A. Plock, “The role of adipose-derived stem cells in breast cancer progression and metastasis,” Stem Cells Int., vol. 2015, p. 120949, 2015. https://doi.org/10.1155/2015/120949
Sekhar, K. C., A. Rajanikanth, M. N. Bobby, and J. R. Kanala, “A review on anticancer potential of natural drugs: Hispolon and limonene,” Int. J. Curr. Microbiol. Appl. Sci., vol. 7, no. 11, pp. 3253–3263, 2018.
Sethulakshmi, K. and R. Lakshmi, “Antibacterial activities of coelomic fluid of local earthworms against disease causing microorganisms,” Asian J. Biol., vol. 5, no. 3, pp. 1–7, 2018.
Sudhakar, A. “History of cancer, ancient and modern treatment methods,” J. Cancer Sci. Ther., vol. 1, no. 2, pp. 1–4, 2009. https://doi.org/10.4172/1948-5956.100000e2
Sun, Z., “Earthworm as a biopharmaceutical: from traditional to precise,” Eur. j. biomed. res., vol. 1, no. 2, pp. 28-35, 2015. https://doi.org/10.18088/ejbmr.1.2.2015.pp28-35
Urry, L. A., M. L. Cain, S. A. Wasserman, P. V. Minorsky, and J. B. Reece, Campbell biology in focus, 2nd ed. Upper Saddle River, NJ: Pearson, 2015.
Wu, S., S. Powers, W. Zhu, and Y. A. Hannun, “Substantial contribution of extrinsic risk factors to cancer development,” Nature, vol. 529, no. 7584, pp. 43–47, 2016. https://doi.org/10.1038/nature16166
Yanqin L. et al., “Coelomic fluid of the earthworm Eisenia fetida induces apoptosis of HeLa cells In vitro,” Eur. J. Soil Biol., vol. 43, pp. S143–S148, 2007. https://doi.org/10.1016/j.ejsobi.2007.08.049
Citations |
 |
 |
 |
 |
