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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 13, Issue:5, May, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(5): 130-136
DOI: https://doi.org/10.20546/ijcmas.2024.1305.018


Association Between Dairy Cow Mastitis Causative Agent and Some Production Parameters using RT-PCR
Mohammed Farah Mohammed Abdelhameed¹*, Mohamed T. Ibrahim¹,Ahmed Eltigani Almansoori² and R. H. Zayed¹
1Department of Dairy Production, College of Animal Production, Sudan University of Science and Technology
2AL Rawabi Dairy Company, Dubai, UAE
*Corresponding author
Abstract:

Mastitis is the term used to describe inflammation in one or more udder parts in dairy cows, which are mostly brought on by bacterial infections that travel within the teat canal; the advancement of microscopes made it possible to identify the microorganisms that cause the majority of mastitis cases, and the process continues until the PCR. The aim of this work is to use RT-PCR to illustrate the association between the mastitis causative agent and some production parameters. In the first few months of 2019, 83 milk samples were aseptically obtained from the herd of 9,000 Holstein milking cows from cows that had been clinically confirmed to have mastitis. To perform the RT-PCR and DNA extraction for each sample, the King Fisher™ Duo Prime Purification System, the Mag MAX™ CORE Nucleic Acid Purification Kit, and the VetMAX™ Masti Type Kit were used. All data were analyzed using the Chi-square test to highlight the association between the causative agent of mastitis and some production parameters. The results indicated a significant association between mastitis and the infected quarter (χ2= 5.06, P=0.024) and between mastitis frequency of previous mastitis infections (χ2= 6.58, P=0.01), lactation number (χ2= 12.55, P=0.01), gestation period (χ2= 32.97, P=0.01), and cow age (χ2= 15.88, P=0.01). Since there are many possible infections and its complicated etiology makes controlling mastitis difficult, more work needs to be put into managing the milking and milking parlor.


Keywords: Mastitis, dairy, prevalence, rt PCR, inflammation


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How to cite this article:

Mohammed Farah Mohammed Abdelhameed, Mohamed T. Ibrahim, Ahmed Eltigani Almansoori and Zayed, R. H. 2024. Association Between Dairy Cow Mastitis Causative Agent and Some Production Parameters using RT-PCR.Int.J.Curr.Microbiol.App.Sci. 13(5): 130-136. doi: https://doi.org/10.20546/ijcmas.2024.1305.018
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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