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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study investigated the in vitro efficacy of various management strategies against Alternaria brassicicola infecting cabbage (Brassica oleracea var. capitata). Aqueous extracts from Barleria prionitis L. and Solanum virginianum L. leaves exhibited concentration-dependent antifungal activity, with Barleria prionitis extract demonstrating the highest inhibition (80.75%) at 6% concentration. GC-MS analysis of the Barleria prionitis extract identified β-sitosterol, oleanolic acid, lupeol, and barlerin, all of which have documented antifungal properties. Similarly, Solanum virginianum extract displayed significant inhibition (82.44%) at 8% concentration, with phytol and solanidine identified as potential antifungal constituents. Commercially available fungicides (Bavistin, Mancozeb, Carbendazim and Captan) also inhibited A. brassicicola growth, with Bavistin exhibiting the strongest activity (100% inhibition at all tested concentrations). Trichoderma viride displayed superior antagonistic potential (83.09% inhibition) compared to Trichoderma harzianum (64.31%) against the pathogen. The findings suggest the potential of botanical extracts and biocontrol agents as eco-friendly alternatives for managing Alternaria brassicicola in cabbage cultivation. Further research is needed to explore the fungicidal mechanisms of identified bioactive compounds and optimize their application strategies for field efficacy.
Ahmad, I., and Ashraf, M. (2016). Fungicidal efficacy of some plant extracts and fungicides against Alternaria brassicae causing Alternaria blight of rapeseed and mustard. Pakistan Journal of Agricultural Research, 27(4), 442-451.
Babu, S., Lavanya, C., and Samiyappan, R. (2000). Evaluation of Trichoderma harzianum and Pseudomonas fluorescens for biological control of Alternaria black spot disease in groundnut. Plant Disease, 84(4), 440-444.
Baskaran, R., Jayaraman, K., and Gowri, S. S. (2018). Optimization of microwave-assisted extraction of bioactive compounds from Murraya koenigii leaves and evaluation of their in vitro antioxidant and anticancer activities. Pharmacognosy Magazine, 14(55), 553-560.
Biswas, T., and Ghosh, S. (2018). In vitro screening of some plant extracts for their antifungal activity against Alternaria brassicicola causing Alternaria blight of mustard. Journal of Applied and Natural Science, 10(2), 568-571.
Choi, N. H., Jang, J. Y., Choi, G. J., Choi, Y. H., Jang, K. S., Nguyen, V. T., Min, B. S., Le Dang, Q., and Kim, J. C. (2017). Antifungal activity of sterols and Dipsacus saponins isolated from Dipsacus asper roots against phytopathogenic fungi. Pesticide biochemistry and physiology, 141, 103–108. https://doi.org/10.1016/j.pestbp.2016.12.006
Chowdhury, S., Mitchell, W. T., and Sicher, R. C. (2013). Alternaria black spot of Brassica rapa: Assessment of disease development and fungicide sensitivity. Plant Disease, 97(11), 1427-1435.
Corlett, M. A., and Mac Latchy, S. P. (1996a). Classification of isolates of Alternaria brassicicola on the basis of cultural characteristics and restriction fragment length polymorphism analysis of a mitochondrial DNA sequence. Canadian Journal of Botany, 74(3), 380-388.
Corlett, M. A., and Mac Latchy, S. P. (1996b). Sequence variation in ribosomal DNA within the genus Alternaria. Mycological Research, 100(8), 961-964.
Dandekar, S. D., Desai, P. V., Gaikwad, S. B., and Giri, A. S. (2015). A validated RP-HPLC method for the quantification of with anolides in Withania somnifera Dunal roots and its herbal formulations. Pharmacognosy Magazine, 11(43), 507-512.
Das, S. N., Paul, P., and Saikia, P. (2023). Efficacy of Fungicides and Botanical Extracts against Alternaria brassicicola Causing Leaf Spot of Cabbage (Brassica oleracea var Capitata L.). Asian Journal of Biological and Life Sciences, 12(3), 493. https://doi.org/10.5530/ajbls.2023.12.65
Do, T. H. T., Pham, T. H., Pham, G. V., Vo, K. A., Nguyen, T. T. T., Vu, D. H., Nguyen, X. C., Vu, V. H., Nghiem, D. T., Choi, G. J., Nguyen, N. H., Nguyen, H. T. Trinh, X. H. and Le Dang, Q. (2022). Potential use of extracts and active constituent from Desmodium sequax to control fungal plant diseases. International Journal of Agricultural Technology 18(2):489-502.
El-Naggar, A. M., Abdel-Rahman, H. M., and Hassan, S. M. (2010). Antifungal activity of Solanum nigrum L. alkaloids. Journal of Applied Microbiology, 108(2), 425-432.
El-Naggar, S. M. Z., and Ghanem, N. M. (2010). Antifungal activity of neo-clerodanediterpenoids from Barleria acanthoides. Journal of Natural Research, 65(5-6), 343-348
Gangaram, S., Naidoo, Y., Dewir, Y. H., and El-Hendawy, S. (2021). Phytochemicals and biological activities of Barleria (Acanthaceae). Plants, 11(1), 82. https://doi.org/10.3390/plants11010082
Goussous, S. J., Abu el-Samen, F. M., and Tahhan, R. A. (2010). Antifungal activity of several medicinal plants extracts against the early blight pathogen (Alternaria solani). Archives of Phytopathology and Plant Protection, 43(17), 1745-1757. https://doi.org/10.1080/03235401003633832
Guerena, M. (2006). Cole crops and other Brassicas: Organic production. ATTRA National Sustainable Agriculture Information Service, Fayetteville, AR, USA, 1-19.
Gupta, A., Naraniwal, M., and Kothari, V. (2012). Modern extraction methods for preparation of bioactive plant extracts. International journal of applied and natural sciences, 1(1), 8-26.
Hajieghrari, B., Mohamadrezaei, M., and Aliasgharzadeh, A. (2008). Antagonistic effect of Trichoderma harzianum against Rhizoctonia solani causing damping-off of melon seedlings. Journal of Plant Protection Research, 48(2), 151-157.
Ishieze, P. U., Amuji, C. F., Ugwuoke, K. I., Baiyeri, P. K., and Eze, M. O. (2023). Comparative Efficacy of Systemic and Combination Fungicides for the Control of Alternaria Leaf Spot of Cabbage. Applied Microbiology, 3(3), 906-914. https://doi.org/10.3390/applmicrobiol3030062
Javed, S., Mahmood, Z., Khan, K. M., Sarker, S. D., Javaid, A., Khan, I. H., andShoaib, A. (2021). Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina. Scientific reports, 11(1), 8417. https://doi.org/10.1038/s41598-021-87725-7
Kavita, K., and Dalbeer, S. (2015). Antifungal efficacy of some plant extracts against Alternaria solani causing early blight of tomato. International Journal of Current Microbiology and Applied Sciences, 4(4), 232-238.
Khalse, S., Kaur, S., and Kapoor, M. (2017). Evaluation of plant extracts for their antifungal activity against Alternaria brassicae infecting mustard (Brassica juncea L.). Journal of Applied Horticulture, 19(2), 142-146.
Khan, I. H., and Javaid, A. (2020). Antifungal activity and GC-MS analysis of n-butanol extract of quinoa (Chenopodium quinoa Willd.) leaves. Bangladesh Journal of Botany, 49(4), 1045-1051. https://doi.org/10.3329/bjb.v49i4.52537
Kithan, C., and Daiho, L. (2014). In Vitro evaluation of botanicals, bio-Agents and fungicides against leaf blight of Etlingera linguiformis caused by Curvularia lunata var. aeria. Journal of Plant Pathology and Microbiology, 5(3), 1. https://doi.org/10.4172/2157-7471.1000232
Kumar, D., Pandey, S., and Singh, M. (2011). Efficacy of Trichoderma harzianum and fungicides against Alternaria blight of Indian mustard caused by Alternaria brassicae. Journal of Mycology and Plant Pathology, 21(2), 221-224.
Kwon, T.-J., Lee, J.-H., and Kim, J.-H. (2003). Antifungal activity of Solanum nigrum L. extracts against Candida albicans. Journal of Microbiology, 41(4), 343-347.
Liu, X., Wang, Y., and Guo, S. (2017). Antifungal activity of triterpenoid acids from Celastrus orbiculatus against Candida albicans. Molecules, 22(3), 434
Madadi A., Rauf, H., H Falahzadah, M., Yousufzai, A., S Jamily, A., and A Sarhadi, W. (2021). Evaluation of in vitro antifungal potential of several fungicides against Alternaria alternata (Fr.) Keissler, the causal agent of potato brown spot in Afghanistan. Novel Research in Microbiology Journal, 5(1), 1106-1117. https://doi.org/10.21608/NRMJ.2021.149383
Meena, M., Swapnil, P., Zehra, A., Dubey, M. K., and Upadhyay, R. S. (2017). Antagonistic assessment of Trichoderma spp. by producing volatile and non-volatile compounds against different fungal pathogens. Archives of Phytopathology and Plant Protection, 50(13-14), 629-648. https://doi.org/10.1080/03235408.2017.1357360
Metz, N., and Hausladen, H. (2022). Trichoderma spp. as potential biological control agent against Alternaria solani in potato. Biological Control, 166, 104820. https://doi.org/10.1016/j.biocontrol.2021.104820
Mukherjee, P. K., Mendoza-Mendoza, A., Zeilinger, S., and Horwitz, B. A. (2022). Mycoparasitism as a mechanism of Trichoderma-mediated suppression of plant diseases. Fungal Biology Reviews, 39, 15-33. https://doi.org/10.1016/j.fbr.2021.11.004
Pun, L. B., Chhetri, K., Pandey, A., and Poudel, R. (2020). In vitro evaluation of botanical extracts, chemical fungicides and Trichoderma harzianum against Alternaria brassicicola causing leafspot of cabbage. Nepalese Horticulture, 14(1), 68-76. https://doi.org/10.3126/nh.v14i1.30612
Rahman, A., and Choudhary, M. I. (2001). Antibacterial and antifungal activities of plant sterols. Natural Product Letters, 15(6), 409-412
Roopa, R., Jeyalakshmi, S., and Kavitha, R. (2014). Antagonistic activity of Streptomyces griseoviridis and Pseudomonas fluorescens against Fusarium oxysporum f. sp. cucumerinum causing wilt of cucumber. Archives of Phytopathology and Plant Protection, 47(16), 1906-1914.
Saharan, M. S., Pattiwar, V. W., and Singh, H. N. (2016). Alternaria leaf blight of crucifers and its management: A review. Plant Pathology Journal, 35(2), 189-209.
Sasode, R. S., Prakash, S., Gupta, A., Pandya, R. K., and Yadav, A. (2012). In vitro study of some plant extracts against Alternaria brassicae and Alternaria brassicicola. Journal of Phytology, 4(1), 44-46.
Shrestha, K., and Tiwari, R. D. (2009). Antifungal activity of some plant extracts against Alternaria solani causing early blight of tomato. Vegetable Science, 36(2), 171-173.
Singh, S., Godara, S. L., and Gangopadhyay, S. (2013). Studies on antifungal properties of plant extracts on mustard blight caused by Alternaria brassicae. Indian Phytopathol, 66, 172-176. https://epubs.icar.org.in/index.php/IPPJ/article/view/29984
Singh, S., Srivastava, R., and Choudhary, S. (2011). Antifungal and high-performance liquid chromatography analysis of the crude extracts ofAcoruscalamus, Tinosporacordifolia and Celestrus paniculatus. Archives of phytopathology and plant protection, 44(9), 902-910.
Tesfaye, B., and Tefera, T. (2017). Extraction of essential oil from neem seed by using soxhlet extraction methods. International Journal of Advanced Engineering, Management and Science, 3(6), 239870.
Thaware, B. S., Thakare, D. D., and Hiware, C. V. (2010). In vitro antifungal activity of Withania somnifera (L.) Dunal extracts against some soilborne phytopathogenic fungi. Bioscience, Biotechnology, and Biochemistry, 74(4), 853-856.
Vincent, J. M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159(4051), 850 https://doi.org/10.1038/159850b0.
Wang, J. S., Wu, J. H., and Chen, C. S. (2005). Antibacterial and antifungal activities of lupeol from Trichodiadema barbatum. Natural Product Research, 19(2), 159-163
Wongamthing, R., and Sainamole Kurian, P. (2023). The effective antagonistic potential of phylloplane microbes against Alternaria solani causing early blight disease in tomato plant. Indian Phytopathology, 76(3), 825-834.
Yadav, S. A., and Koshi, F. S. (2022). Phytochemicals from Solanaceae family and their anticancer properties. In Medicinal Plants. IntechOpen. https://doi.org/10.5772/intechopen.104462
Yu, X. (2015). A monograph of Alternaria species in China. Science Press Beijing and Higher Education Press Beijing.
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