National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The ESBL producing isolate of E.coli among other clinical isolates has been increasing over the past few years resulting in limitation of therapeutic options. Bacteria produces ESBL poses a major problem for clinical therapautics. A cross sectional study was carried out in the Department of Microbiology Sharda Hospital School of Medical Science and Research, Sharda University, Greater Noida. 204 E.coli were isolated from various clinical samples which consists of urine, pus, sputum, blood, stool, swab, and different body fluids from both IPD & OPD patents were included in this study. Sample were processed & identified as per routine laboratory protocol. ESBL screening & confirmatory along with AST was done by Kirby - Bauer disc diffusion method. The following antibiotic disc were used Cefotaxime, Ceftriaxone, Ceftazidime, Ampicillin, Meropenem, Fosfomycin, Tetracycline, Cefepime, Cotrimoxazole, Nalidixic acid according to the clinical laboratory standard institution (CLSI) guideline 2019. The most common isolates were found from urine 153 (75%) followed by Pus 27 (13%). In the present study used two different confirmatory methods for the detection of ESBL producing E.coli. Combined disc test & Double disk synergy test. During the study period of total no. of E.coli isolates were 204, out of which 168 E.coli isolates were resistant to 3rd generation cephalosporin,(51%) isolates were ESBL producer by double disc synergy test & (55%) isolates were ESBL producer by combined disc test. ESBL producer of E.coli isolates showing a great degree of resistance to antibiotics. The study reveals higher percentage of isolates were resistant to 3rd generation cephalosporin and ESBL producer were more. This study conclude that resistant to cephalosporin were due to extended spectrum beta lactamase production in our isolates & Combined disk test was found to be a better test as compared to double disk synergy test.
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