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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 13, Issue:1, January, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(1): 73-80
DOI: https://doi.org/10.20546/ijcmas.2024.1301.009


Magnetic Beads Carried Over in Extracted DNA Elution from Mastitis Cow Milk
Mohammed Farah Mohammed Abdelhamed¹, Rania Hassan Zaid¹,Mohamed T. Ibrahim¹ and Ahmed Eltigani Almansoori²
1Department of Dairy Production, College of Animal Production,
Sudan University of Science and Technology, Sudan
2AL Rawabi Dairy Company, Dubai, UAE
*Corresponding author
Abstract:

Using the magnetic separation technique in nucleic acid extraction is considered one of the most rabid and easy automated extraction methods that use minimal amounts of equipment. This paper describes some cow-related factors that may affect the carrying of magnetic beads during the extraction process. A total of 85 milk samples were collected from clinically infected cows with mastitis. DNA extraction is done using the Mag MAX™ CORE Nucleic Acid Purification Kit and the King Fisher™ Duo Prime Purification System. Fifty-three (62.4%) samples are extracted normally, while thirty-two (37.6%) have a magnetic bead carried over, and two (6.3%) out of the thirty-two supernatant DNA samples can’t be collected. To evaluate the impact of lactation, frequency of mastitis, days in milk, and gestation period on magnetic beads carried over during DNA extraction when using the magnetic separation technique, a Chi-square test of independence was performed, and it showed that there was no significant association between magnetic beads carried over and lactation (Chi square = 0.28, P = 0.60), frequency of mastitis (Chi square = 2.26, P = 0.13), days in milk (Chi square = 0.84, P = 0.66), and gestation period (Chi square = 4.24, P = 0.12). Therefore, more studies need to be conducted to interpret this phenomenon.


Keywords: Magnetic beads, lactation, days in milk, gestation period, geochemical conditions


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How to cite this article:

Mohammed Farah Mohammed Abdelhamed, Rania Hassan Zaid, Mohamed T. Ibrahim and Ahmed Eltigani Almansoori. 2024. Magnetic Beads Carried Over in Extracted DNA Elution from Mastitis Cow Milk.Int.J.Curr.Microbiol.App.Sci. 13(1): 73-80. doi: https://doi.org/10.20546/ijcmas.2024.1301.009
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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