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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 13, Issue:1, January, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(1): 61-67
DOI: https://doi.org/10.20546/ijcmas.2024.1301.007


Molecular Characterization of Azotobacter and PSB from Xerophytic Plants of Maharashtra State, India
Monika Suresh Barge* and T. K. Narute
Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413722, India
*Corresponding author
Abstract:

The present study was conducted at Department of Plant Pathology and Agricultural Microbiology, MPKV, Rahuri during year 2021 to 2023 to characterize and screen different isolates of Azotobacter and PSB from Different Xerophytic plants from Different districts of Maharashtra State. 68 Azotobacter isolates were biochemically characterized and screened under In vitro conditions for their plant growth promoting properties. This isolates were biochemically characterized and screened In-vitro condition for their plant growth promoting properties. DNA polymorphism of isolates was studied using randomly amplified polymorphic DNA analysis. The genetic diversity among the bacterial strains was assessed by RAPD analysis. All the isolates showed reproducible DNA banding pattern. Diversity among the bacterial strains was assessed on the basis of variation of size number and intensity of bands.


Keywords: RAPD primer, Genetic diversity, OPB marker, PCR Amplification


References:

Achenbach L. A, Patric J, Gray. Use of RAPD markers as a diagnostic tool for the identification of Fusarium solani isolates that cause Soybean sudden death syndrome. Plant dis. 1996; 80: 1228-1232. https://doi.org/10.1094/PD-80-1228

Babalola., Osir E O and Sanni A I 2002. Molecular techniques: An overview of methods for the detection of Bacteria 2(12) : 710-713.

Hafeer, F. Y., Sumera, Y., Dini Ariani, Non Renseigné, Yusuf Zafar and Kauser, A. M. 2006. Plant growth promoting bacteria as biofertilizer. Agronomy for Sustainable Develop.26(2) : 143-150. https://doi.org/10.1051/agro:2006007

Hebb, D., Richardson, M. A. E., Reid, R. and Brockwell, J. 1998. PCR as an ecological tool to determine the establishment and persistance of Rhizobium strains introduced into the field as seed inoculants Diane. Australian J. Agric. Res. 49 : 923-34. https://doi.org/10.1071/A98007

Longanathan, P., Sunita, R., Parida, A. K and Nair, S. 1999. Isolation and characterization of two genetically distant groups of Acetobacter diazotrophicus from a new host plant Elusine carcana L. J. Applied Microbiol., 87 : 167-172. https://doi.org/10.1046/j.1365-2672.1999.00804.x

Sharma et al., (1999) PCR used to distinguish very closely related strain of pathogen without prior knowledge of the nature of polymorphic region by random amplified polymorphic

Williams, J. G., Kubelic, A. R., Livak, K. J., Rafalaski, J. A. and Tngey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic Markers. Nucleic acids Res.18(22) : 6531-6535. https://doi.org/10.1093/nar/18.22.6531

 


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How to cite this article:

Monika Suresh Barge and Narute, T. K. 2024. Molecular Characterization of Azotobacter and PSB from Xerophytic Plants of Maharashtra State, India.Int.J.Curr.Microbiol.App.Sci. 13(1): 61-67. doi: https://doi.org/10.20546/ijcmas.2024.1301.007
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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