Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer


See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 12, Issue:11, November, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(11): 68-73
DOI: https://doi.org/10.20546/ijcmas.2023.1211.008


Extraction and Stability Test of Betacyanin Pigment in Dragon Fruit Skin (Hylocereus polyrhizus) as an Alternative to 2% Eosin Dyes in Examination of Soil Transmitted Helminth Worm Eggs
Yanti Rahayu* and T. Arniat Christiani
Medical Laboratory Technology,, Institute of Health Science, Syedza Saintika, Indonesia
*Corresponding author
Abstract:

Worm eggs are revealed to be colorless upon direct inspection, making them challenging to observe under a microscope. An easy technique for studying worm eggs is to utilize Eosin 2%, which is typically used in medical research and diagnostics. Eosin has some drawbacks, including a high price tag and environmentally hazardous ingredients. Consider using the skin of dragon fruits as a source of dye. The red pigment betacyanin gives dragon fruit skin its color. The intention was to determine if intestinal nematode worm egg coloration could be substituted with dragon fruit peel (Hylocereus polyrhizus). The red dragon fruit skin was extracted with ethanol as a solvent and 2% eosin as a reference to create the dye for this experimental study. Using ethanol solvent to dye feces preparations from dragon fruit peel extract demonstrates the possibility of doing so. Extract from the peel of dragon fruit shows promise as a coloring agent. As a result, 2% eosin can be replaced with dragon fruit peel extract (Hylocereus polyrhizus) when studying worm eggs.


Keywords: Soil transmitted helminths, dragon fruit skin, betacyanin

Download this article as Download

How to cite this article:

Yanti Rahayu and Arniat Christiani, T. 2023. Extraction and Stability Test of Betacyanin Pigment in Dragon Fruit Skin (Hylocereus polyrhizus) as an Alternative to 2% Eosin Dyes in Examination of Soil Transmitted Helminth Worm Eggs.Int.J.Curr.Microbiol.App.Sci. 12(11): 68-73. doi: https://doi.org/10.20546/ijcmas.2023.1211.008
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations