National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Mangifera caesia plant was a plant from Kalimantan which was now rarely found, due to the pathogenic fungi infection found on the leaves, thus it was able to obstruct photosynthesis process on the plant. One of attempts to hamper this pathogenic fungi growth was to present antagonistic fungi. The aims of this research: (1) to identify the highest antagonism effect of Trichoderma harzianum and T. viride, (2) to analyze the amount of enzyme produced from Trichoderma harzianum and T. viride, (3) to examine antagonism mechanism of Trichoderma harzianum and T. viride on Collectotrichum gloeosporioides fungi based on microscopic examination. The research method was multiple culture method inoculating antagonistic fungi: Trichoderma harzianum and T. viride also pathogenic fungi: Colletotrichum gloeosporioides on media of paired PDA plate, and then incubated at 250-270C for 4x24 hours. Next, the antagonism effect of Trichoderma harzianum and T. viride on Colletotrichum gloeosporioides, and the mechanism of antagonism would be examined by Scanning Electron Microscope (SEM), the results were analyzed descriptively and quantitatively. This research results referred that: (1) antagonistic fungi of Trichoderma harzianum has the highest antagonism power with the average percentage of antagonism 78,39% rather than T. Viridae fungi on Colletotrichum gloeosporioides, (2) cellulose enzyme produced by Trichoderma harzianum was greater, around 143,562 U/,L than T. Viridae fungi, (3) antagonism mechanism of Trichoderma spp. indicated that three mycoparasitism mechanisms in three methods: sticking, twisting, and piercing on hyphae fungi of Colletotrichum gloeosporioides.
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