National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cellulose degrading bacteria plays a crucial role to convert cellulose into valuable products through cellulase enzyme. In this study, 21 bacteria were isolated from dump site of sewage pumping station, Khajod, Gujarat, India. Amongst, three bacteria viz., SWCDB6 (1.32; 0.47; 0.36 and 0.46), SWCDB21 (1.27; 0.55; 0.36 and 0.48) and SWCDB5 (1.20; 0.52; 0.33 and 0.43) showed potent Cellulolytic Index (CI), CMCase (U/ml) activity, FPase (U/ml) activity and cell biomass (OD660), respectively at 72 hrs at 30 ºC ± 0.2. Effect of varied pH, temperature, incubation period, carbon sources (starch and cellulose) and nitrogen source (ammonium sulphate and urea) studied to optimize the maximum cellulase production by these three isolates. Maximum CMCase, FPase and biomass production was recorded with pH 7.0 at 35 ºC ± 0.2 at 96 hrs along with addition of 0.5 % ammonium sulfate and starch in the medium for all three isolates. However, SWCDB21 was found to be potent cellulase producer and molecularly identified as Staphylococcus species. It can be further explored for agriculture waste degradation after thorough in vivo studies.
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