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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:9, September, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 3061-3068
DOI: https://doi.org/10.20546/ijcmas.2019.809.350


Evaluation of Antioxidant and Antibacterial Activities of Emblica officinalis (Amla)
A. Akhila1 and E. Keshamma2*
1Department of Zoology and Genetics, Government Science College, Nrupathunga Road, Bengaluru, Karnataka, India 2Department of Biochemistry, Maharani Science College for Women, Palace Road, Bengaluru, Karnataka, India
*Corresponding author
Abstract:

E. officinalis (Amla) enjoys a holy position in Ayurveda-an Indian indigenous system of medicine. Hence, in the current study we aimed for determination of antioxidant and antibacterial potential of Emblica officinalis (Amla). Leaves of E. officinalis was subjected to successive solvent extraction by continuous hot extraction (Soxhlet) with double distilled water. Antioxidant assay was carried out by in-vitro model using DPPH free radical scavenging activity. In-vitro antibacterial activity of aqueous (Aq.) extract of leaves of E. officinalis (Amla) was assessed using agar well diffusion method with Chloramphenicol as positive control and Dimethyl sulfoxide (DMSO) as negative control. The zones of inhibition after 48 hours of was measured in millimeters (mm). Results delineated that Aq. extract of leaves of E. officinalis (Amla) showed a dose dependent DPPH scavenging activity. Furthermore, the DPPH free radical scavenging activity of Aq. extract of leaves of E. officinalis (Amla) were comparable with that of standard ascorbic acid. In addition, Aq. extract of leaves of E. officinalis (Amla) possess potential antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsella pneumoniae, and methicillin resistant Staphylococcus aureus (MRSA). In conclusion, E. officinalis could be used alternative to synthetic antioxidants and antimicrobials since Aq. extract of leaves of E. officinalis mimic the biological activities of synthetic antioxidant and antimicrobials.


Keywords: Emblica officinalis, Antioxidant, Antibacterial, K. pneumoniae, S. aureus, E. coli

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How to cite this article:

Akhila, A. and Keshamma, E. 2019. Evaluation of Antioxidant and Antibacterial Activities of Emblica officinalis (Amla).Int.J.Curr.Microbiol.App.Sci. 8(9): 3061-3068. doi: https://doi.org/10.20546/ijcmas.2019.809.350
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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