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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:6, June, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(6): 76-89
DOI: https://doi.org/10.20546/ijcmas.2023.1206.010


Predominance of Wild K13 Propeller Haplotypes in three localities of Côte d'Ivoire one decade after the adoption of Therapeutic Combinations based on Artemisinin Derivatives
Dagnogo Oléfongo1,2*, Kipré Gueyraud Rolland1, Ako Aristide Bérenger2, Bla Kouakou Brice1, Touré Offianan André2 and Djaman Allico Joseph1,2
1Unité de Formation et de Recherche (UFR) Biosciences, Laboratoire de Biologie et Santé,Université Felix Houphouët-Boigny, BP V 34 Abidjan 01, Côte d’Ivoire
2Institut Pasteur de Côte d'Ivoire, Département de Parasitologie -Mycologie. 01 BP 490 Abidjan 01, Côte d'Ivoire
*Corresponding author
Abstract:

Background: The emergence of P. falciparum resistance to artemisinin-based combination therapy (ACT) threatens malaria control in Africa. The monitoring of polymorphisms in molecular markers associated with antimalarial drug resistance is essential for malaria control and elimination efforts. The purpose of this study is to analyze the polymorphism of the pfK13 propeller gene of P. falciparum to artemisinin-based combination therapy (ACT) in three sites in southern Côte d'Ivoire. Methods: After obtaining informed consent, blood samples were collected from patients of any sex and aged over 2 years with uncomplicated P. falciparum malaria in Anonkoua-Kouté, Port-Bouët and Ayamé. P. falciparum genomic DNA was extracted and amplified by Nested-PCR using Pfk13 propeller gene-specific primers. The amplification products were sequenced using the Sanger method at Eurofins Genomics. The key codons (493, 539, 543 and 580), molecular markers of the resistance of P. falciparum to ACTs and the additional codons (476 and 561) were analyzed. Results: A total of 186 DNA extracts from blood samples collected from the three study sites were amplified and sequenced. Of the 93 PCR products sequenced, 84.94% (158/186), 91.39% (170/186), 95.69% (178/186), 100% (186/186), 82.79% (154/186) and 94.62% (178/186) corresponding to codons 493, 539, 543, 580, 476, 561, respectively, were successfully analyzed. Thus, the prevalences of wild-type alleles were 92.40% (Tyr493), 94.11% (Arg539), 94.38% (Ile543), 91.39% (Cys580), 89.61% (Met476) and 88.63% (Arg561) against 1.26% (His493), 3.52% (Thr539) and 1.29% (Ile476) of mutant alleles. The mutant alleles Thr543, Tyr580, and His561 were not observed in all three study sites. The analysis indicated a predominance of the YRICMR allelic form representing a susceptible haplotype (78.49%). No significant difference was observed between the prevalences of the wild-type alleles determined in the three study sites (p>0.05). Conclusion: More than a decade after the adoption of ACTs for the management of uncomplicated malaria, these combinations are still effective in Anonkoua-Kouté, Port-Bouët and Ayamé in the south of Côte d'Ivoire.


Keywords: Artemisinin-based combination therapy, Côte d'Ivoire, pfK13 propeller, Resistance

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How to cite this article:

Dagnogo Oléfongo, Kipré Gueyraud Rolland, Ako Aristide Bérenger, Bla Kouakou Brice, Touré Offianan André and Djaman Allico Joseph. 2023. Predominance of Wild K13 Propeller Haplotypes in three localities of Côte D'ivoire one decade after the adoption of Therapeutic combinations Based on Artemisinin Derivatives.Int.J.Curr.Microbiol.App.Sci. 12(6): 76-89. doi: https://doi.org/10.20546/ijcmas.2023.1206.010
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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