International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:3, March, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(3): 243-249

Isolation and Characterization of Oleaginous Yeast
N. R. Akash1 and G. D. Goudar2
1Department of Agricultural Microbiology, College of Agriculture, Dharwad, Karnataka, India
2Senior Microbiologist AICRP on EAAI (Bioconversion) MARS, University of Agricultural Sciences, Dharwad, Karnataka, India
*Corresponding author

Oleaginous yeast are known to synthesize and accumulate large amount of intracellular triacylglycerol. Hence, they are considered to be one of the most promising alternate sources for oil production. In this regard, the present study was undertaken to isolate, characterize and screen oleaginous yeast for biomass and lipid production. Eighty yeast isolates were obtained from 19 soil samples collected from different districts of Karnataka. Out of 80 isolates, ten isolates were confirmed to be oleaginous based on Sudan Black B staining. These isolates were further analyzed for biomass and lipid production. The reference strain Debaryomyceshansenii var. hansenii was also used in the study. The highest biomass and lipid production of 806.03 mg/100ml and 123.51 mg/100ml respectively was recorded by the isolate OYB-1 followed by OYH-2 with biomass production of 790.59 mg/100ml and lipid production of 120.50 mg/100ml. Hence the isolate OYB-1 can be exploited for biodiesel production.

Keywords: Oleaginous yeast, Lipid content, Biomass, Biodiesel

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How to cite this article:

Akash, N. R. and Goudar, G. D. 2021. Isolation and Characterization of Oleaginous Yeast.Int.J.Curr.Microbiol.App.Sci. 12(3): 243-249. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.