'' '' '' Improvement of Fibrinolytic Enzyme Production from <em>Bacillus</em> sp. ES4 by Response Surface Methodology and Exponential Fed-Batch Fermentation
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles                      Volume : 12, Issue:3, March, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(3): 151-162
DOI: https://doi.org/10.20546/ijcmas.2023.1203.019


Improvement of Fibrinolytic Enzyme Production from Bacillus sp. ES4 by Response Surface Methodology and Exponential Fed-Batch Fermentation
1School of Biotechnology and Food Technology,
2Laboratory of Applied Microbiology, Hanoi University of Science and Technology, Hanoi, Vietnam
*Corresponding author
Abstract:

To improve the fibrinolytic enzyme production of the mutant strain Bacillus sp. ES4, the response surface methodology was used to optimize the medium composition. Thirty-four flask scale experiments were carried out, and the results were fitted to quadratic models using Design-Expert software. The ANOVA analysis revealed that medium components differently affect cell density and fibrinolytic enzyme production. At optimal condition for fibrinolytic enzyme production, enzymatic activity attained 686 ± 43 FU/mL, which is increased by 1.7-fold compared to the initial medium (404 ± 4 FU/mL). One step fed-batch fermentation, in which the nutrient for optimal enzymatic activity was exponentially feed to the culture, improved fibrinolytic enzyme activity by 5.91-fold (4,057.14±57.14 FU/mL) and cell density by11.9-fold (101.2±0.005). In two steps fed-batch fermentation, the nutrient for optimal cell density was first used, then followed by feeding for optimal enzyme activity. The highest cell density (OD600nm) and fibrinolytic enzyme activity reached 142.3 ± 0.0125 and 5,300 ± 100 FU/mL, respectively after 13 h fermentation. Ultimately, the medium optimization and fed-batch strategy collectively improved fibrinolytic enzyme activity by 13.12-fold.


Keywords: Fibrinolytic enzyme, response surface methodology, exponential fed-batch fermentation
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How to cite this article:

Bui Thi Thanh, Le Tuan, Nguyen Lan Huong and Pham Tuan Anh. 2023. Improvement of Fibrinolytic Enzyme Production from Bacillus sp. ES4 by Response Surface Methodology and Exponential Fed-Batch Fermentation.Int.J.Curr.Microbiol.App.Sci. 12(3): 151-162. doi: https://doi.org/10.20546/ijcmas.2023.1203.019
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.