International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:2, February, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(2): 232-245

Detection of Genes involved in the Biosynthesis of Aflatoxin B1 in Aspergillus spp strains isolated from Moringa oleifera powder marketed in Abidjan (Côte d'Ivoire)
Yakoura Karidja Ouattara1, 2*, Kalpy Julien Coulibaly2, Amine NatyTadiogo Kone1,Sylvie Mireille Kouame-Sina3, Sylla Aboubacar3, Karidja Thanon4, Eugene Patrice Kissiedou2, Andre Offianan Toure4, Adjehi Dadie1 and Mireille Dosso2
1Departement of Food Sciences and Technologies (STA), Laboratory of Microbiology and Biotechnology, Nangui Abrogoua University, Abidjan, Côte d’Ivoire
2Department of Environment and Health,
3Department of Molecular Biology,
4Department of Mycology and Parasitology, Pasteur Institute of Côte d’Ivoire, Abidjan, Côte d’Ivoire
*Corresponding author

Moringa oleifera, traditionally consumed in some countries, is now widely distributed throughout the tropics, particularly in Africa. It is consumed in the form of food supplements in Côte d'Ivoire. However, the production method of Moringa leaf powder could be a source of contamination by potentially mycotoxigenic moulds. The objective of this work was to evaluate the level of contamination of Moringa powder by Aspergillus Spp strains and to detect the genes involved in the biosynthesis of aflatoxin B1. A total of 288 samples of Moringa powder were collected from vendors in six communes of Abidjan district. Isolation and purification of Aspergillus isolates were performed on Sabouraud medium with chloramphenicol. The identification of these strains was carried out by macroscopic and microscopic observations. The molecular aspect of the Aspergillus species and the detection of the Nor, Ver, AvfA and AflR1 genes, involved in the biosynthesis process of aflatoxin B1, were carried out by PCR. The results show that the contamination rate of Moringa powder by Aspergillus spp was 62.46% with a predominance of Aspergillus niger (33.16%). Other species namely Aspergillus flavus (27.07%), Aspergillus fumigatus (24.87%) and Aspergillus terreus (13.98%) were also observed. The prevalences of strains harbouring the AflR1, Nor, Ver, and AvfA genes were 70.47%, 73.07%, 52.85% and 41.97% respectively. Strains harbouring AflR, Nor, Ver, and AvfA genes simultaneously were also detected in proportions of 39.37%. The presence of Aspergillus harbouring the genes coding for Aflatoxin B1 biosynthesis in Moringa powder could constitute a health risk for the consumer.

Keywords: Aflatoxin, Aspergillus spp, Moringa oleifera, leaves, preservation and consumption

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Yakoura Karidja Ouattara, Kalpy Julien Coulibaly, Amine NatyTadiogo Kone, Sylvie Mireille Kouame-Sina, Sylla Aboubacar, Karidja Thanon, Eugene Patrice Kissiedou, Andre Offianan Toure, Adjehi Dadie and Mireille Dosso. 2023. Detection of Genes involved in the Biosynthesis of Aflatoxin B1 in Aspergillus spp strains isolated from Moringa oleifera powder marketed in Abidjan (Côte d'Ivoire).Int.J.Curr.Microbiol.App.Sci. 12(2): 232-245. doi:
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